(3-chloro-2-hydroxypropyl)dimethyloctadecylammonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(3-chloro-2-hydroxypropyl)dimethyloctadecylammonium chloride

EC Number:

221-083-1

EC Name:

(3-chloro-2-hydroxypropyl)dimethyloctadecylammonium chloride

Cas Number:

3001-63-6

Molecular formula:

C23H49ClNO.Cl

IUPAC Name:

(3-chloro-2-hydroxypropyl)dimethyloctadecylazanium chloride

Constituent 2

Reference substance name:

Propane-1,2-diol

EC Number:

200-338-0

EC Name:

Propane-1,2-diol

Cas Number:

57-55-6

Molecular formula:

C3H8O2

IUPAC Name:

propylene glycol

Test material form:

liquid

Details on test material:

Aqueous solution

Test solutions

Vehicle:

yes

Details on test solutions:

100 mg of QUAB 426 was accurately weighed out and dissolved in 1000 ml of dilution water. From this solution 1.0, 1.8, 3.2, 5.6 and 10 ml were taken
and diluted to one Litre with dilution water. These solutions, containing 0.1, 0.18, 0.32, 0.56 and 1.0 mg/l were used as test solutions.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The organism used was the fresh-water fish species Brachydanio rerio cultured in the Laboratory. The test animals were 1.7 +/- 0.2 cm long and weighed 0.04 +/- 0.01 g.

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

Expressed as CaCo3, approx 210 mg/l

Test temperature:

Test solutions were kept at 24°C +/- 1°C

pH:

The pH of the test and control solutions varied between 8.0 and 8.3.

Dissolved oxygen:

The oxygen concentrations of the test and control solutions were greater than 7.3 mg.

Details on test conditions:

the five concentrations of the test substance were tested. The test was performed in 1000 ml all-glass beakers , each containing 1000 ml of test solution. Two beakers containing 5 fishes each were used for each test or control solution. The test lasted 96 h. The test and control solutions were renewed daily. The fishes were not fed. At the beginning and at the end of the test and at each renewal time the pH and the oxygen concentration of the test and control solutions were measured. The test solutions were kept at 24 +/- °C and slightly aerated. The dead animals were counted and removed at least after 24 h, 48 h, 72 h and 96 h. At the same times the condition of the survivors was compared with that of the control animals in the blanks.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 0.34 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.18 mg/L

Conc. based on:

test mat.

Basis for effect:

behaviour

Applicant's summary and conclusion

Conclusions:

The LC50 (96h) of the test solution was found to be 0.34 mg/l. Based on a concentration of the substance of 40 %, the LC50 of 3-Chloro-2-hydroxypropyldimethyloctadecylammoniumchloride woudl be 0.85 mg/l.