3-methylpent-1-en-3-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. However, no information on the preparation of the test media was given, but scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 79/831 EWG, Attachment V, C2

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-en-2-ol

EC Number:

204-068-4

EC Name:

2-methylbut-3-en-2-ol

Cas Number:

115-18-4

Molecular formula:

C5H10O

IUPAC Name:

2-methylbut-3-en-2-ol

Details on test material:

- Name of test material (as cited in study report): 2-methylbuten-3-ol-2
- Lot/batch No.: 88/70
- Analytical purity: > 99 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the test item of the highest test concentration (nominal 500 mg/L) was prepared. Then, adequate volumes of this stock solution were diluted with culture medium to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 +/- 0.50 mmol/l

Test temperature:

19 - 21 °C

pH:

8.0 to 8.1

Dissolved oxygen:

8.5 to 8.9 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
control,
62.2,
125,
250,
500

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes with flat bottom (glass)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water :A synthetic fresh water was used for culture and test purposes. Properties of this medium: Ratio Na:K: 10 : 1; pH value: 7.7 - 8.3. After preparation the medium was aerated for approximately 24 h until saturation.
- Alkalinity: up to pH 4.3: 0,80 +/- 0.10 mmol/l
- Ca/mg ratio: 4.1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 light : 8 h dark
- Light intensity: Artificial light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Swimming ability of the test animals after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The number of mobile daphnids after hours of exposure at the different  test concentrations was as follows:

Concentration   Mobile daphnia after x hours
[mg/l]          0       3       6       24       48
Control        20      20      20      20       20
62.5           20      20      20      20       20  
125            20      20      20      20       20 
250            20      20      20      19       19       
500            20      20      20      20       20

The calculated EC50 (48 h) was > 500 mg/l (confidence limits 95%: not  determined). The EC0 (48 h) was 500 mg/L and the EC100 (48 h) was > 500  mg/L.

The calculated EC50 (24 h) was > 500 mg/l (confidence limits 95%: not  determined). The EC0 (24 h) was 500 mg/L and the EC100 (24 h) was > 500  mg/L.

All results refer to nominal test concentrations.