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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

In order to assess the biodegradation of the test substance, a screening test was performed according to OECD TG 301D (Closed Bottle test) and under GLP conditions. In this study activated sludge was exposed to 2.0 mg/L of the test substance for 60 days. Inhibition of the endogenous respiration of the inoculum by the test substance at day 7 was not detected. The test substance was biodegraded for 27% at day 28 in the Closed Bottle test and should therefore not be classified as readily biodegradable. In the prolonged Closed Bottle test (enhanced biodegradability test) the test substance was only biodegraded by 42% at day 60 which does not allow a classification as not persistent. The test is valid as shown by an endogenous respiration of 0.8 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 81% of its theoretical oxygen demand after 14 days. The oxygen concentrations were >0.5 mg/L in all bottles during the test period.

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable

Additional information