3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at the time of study conduct.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

EC Number:

211-477-1

EC Name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

Cas Number:

647-42-7

Molecular formula:

C8H5F13O

IUPAC Name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

Details on test material:

- Purity: 99.7%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: One sample plus a back-up sample of each test concentration including the dilution water control on day 0 of the test before the test solutions were poured into the replicate test chambers. One sample plus a back-up sample of all test concentrations including the dilution water control at test end (day 2).
- Sampling method: High performance liquid chromatography with detection by mass spectrometry (LC/MS/MS).
- Sample storage conditions before analysis: Refrigerated upon receipt and when not in use.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION: Test substance solutions were prepared by dilution from a stock solution of the test substance in testing facility well water. A 10 mg/L stock solution was prepared by adding approximately 3.25 μL (1.60 mg test substance per μL) of test material to testing facility well water in a 500 mL volumetric flask filled to zero headspace (final volume approximately 520 mL), sonicating for approximately 20 minutes, stirring for approximately 20 minutes, sonicating again for approximately 20 minutes, and stirring again for approximately 35 minutes. The stock solution was also the highest test concentration.

Test solutions were prepared by adding the appropriate volume of the stock solution to the appropriate volume of testing facility well water in 100 mL volumetric flasks filled to zero headspace (final volume approximately 110 mL) and stirring for approximately 5-10 minutes. The stock solution (and highest test solution) was clear and colourless with no visible precipitate. The test solutions were clear and colourless with no visible precipitate.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM:
- Age at study initiation: < 24 hours; collected from the 3rd and 6th broods of 14- and 20-day old parent daphnids.
- Feeding during test: None
- Method of breeding: Daphnids were reared at testing facility in 1000-mL Pyrex® beakers (10 per beaker) which contained 1000 mL of testing facility well water held at 20 ± 2°C. Daphnids were fed daily Monday through Friday with 2.4 mL/L of a yeast, cereal leaves, and trout chow (YCT) mixture and the green alga, Pseudokirchneriella subcapitata, at a rate of approximately 62500 cells/mL of culture media. The YCT mixture was standardised to 1700-2100 mg/L solids. The combination of YCT and alga is equivalent to approximately 0.1-0.2 mg total organic carbon per daphnid per day.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

Total alkalinity (dilution water control), 47 mg/L as CaCO3; hardness( dilution water control), 137 mg/L as CaCO3. To maintain test concentrations, alkalinity and hardness were not taken in the test substance test chambers.

Test temperature:

20.1 to 20.4°C (dilution water control). To maintain test concentrations, temperature measurements were not taken in the test substance test chambers.

pH:

7.2 to 7.6 (dilution water control). To maintain test concentrations, pH measurements were not taken in the test substance test chambers.

Dissolved oxygen:

To maintain zero headspace, dissolved oxygen measurements were not taken.

Nominal and measured concentrations:

Nominal concentrations: 0, 0.625, 1.25, 2.50, 5.00, and 10.0 mg/L
Nominal concentrations adjusted for purity: 0, 0.623, 1.25, 2.49, 4.99, and 9.97 mg/L
Mean Measured concentrations: 0, 0.600, 1.23, 2.39, 4.90, and 9.29 mg/L
Mean, measured values ranged from 93 to 98% of the targeted nominal test concentrations adjusted for test substance purity of 99.7%

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass vials (44-mL). The test chambers were covered with modified lids containing a septum during the test. The modified lids were used to ensure zero-headspace in the test chambers. A zero-headspace chamber was used to ensure the maintenance of test concentrations for the
duration of the study.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Conductivity: 270 μmhos/cm(dilution water control). To maintain test concentrations, conductivity measurements were not taken in the test substance test chambers.

OTHER TEST CONDITIONS
- Photoperiod: A photoperiod of 16 hours light and 8 hours darkness was employed, which included 30 minutes of transitional light (15-19 Lux) preceding and following the 16-hour light interval.
- Light intensity: 495-534 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility (lack of reaction to application of a gentle stimulus); Observations were made daily.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.84 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

other: immobility

Remarks on result:

other: 6.75 to 9.39 mg/L (95% CL)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.39 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

other: immobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

9.29 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

other: immobility

Details on results:

- Behavioural abnormalities: Lethargy was observed in surviving daphnids in the 2.39 (3 of 20), 4.90 (3 of 15), and 9.29 mg/L (5 of 7) mean, measured test concentrations at the end of the study.

Reported statistics and error estimates:

The 48-hour EC50 value was calculated by probit analysis based on mean, measured concentrations of the test substance and immobility. The highest mean, measured concentration causing no immobility at test end and the lowest mean, measured concentration causing 100% immobility at test end were assessed by visual observation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
48-hour EC50 = 7.84 mg/L

Executive summary:

Exposure of daphnids to mean, measured test substance concentrations of 0.600, 1.23, 2.39, 4.90, and 9.29 mg/L resulted in 0, 0, 0, 25, and 65% immobility, respectively, at the end of 48 hours. Mean, measured concentrations ranged from 93 to 98% of the nominal concentrations. No immobility or sublethal effects were observed in the dilution water control daphnids. The highest mean, measured concentration causing no immobility at test end was 2.39 mg/L. The lowest mean, measured concentration causing 100% immobility at test end was greater than 9.29 mg/L. Mean, measured concentrations of the test substance were used for calculation of EC50 values. The 48-hour EC50, based on mean, measured concentrations of the test substance and immobility, was 7.84 mg/L.