Reaction products of diazotised 3-amino-2-hydroxy-5-nitrobenzenesulphonic acid, coupled with 1,3-diaminobenzene and diazotised sodium 4-aminobenzenesulfonate, metallised with Basic Chromium (III) Sulphate

Endpoint summary

Description of key information

Short-term toxicity to fish

LC50 (48 h, Oncorhynchus mykiss) = 700 mg/L nominal conc.

Short-term toxicity to aquatic invertebrates:

EC50 (Daphnia magna, 48h) =213,60 mg/L nominal conc.

Toxicity to aquatic plants other than algae:

ErC50(Lemna minor, 7d) >100 mg/L nominal conc.

Toxicity to microorganisms

IC50 (3 h) >100 mg/L nominal conc.

Additional information

Firstly, it is worth saying that the substance is very water soluble (i.e. > 10 g/L); it has a log Kow lower than 4 (indicating a low potential for bioaccumulation) and is not rapidly degradable.

Short-term toxicity to fish

The substance was tested for acute toxicity toOncorhynchus mykissat the nominal concentrations of 100, 200, 500 and 1000 mg/L during a study period of 48 h.

The LC50 (48 h,Oncorhynchus mykiss) was graphically determined to be 700 mg/L.

Short-term toxicity to aquatic invertebrates

A static test was performed according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation (EC) No. 440/2008. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

Three concentrations showed significant toxicity between 10 and 40 % immobilisation. None of the animals was immobilised in the blank control.

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usinga photometer. The concentrations determined at the start of the test were between 95 % and 97 % of the nominal concentrations. At the end of the test the determined concentrations were between 110 % and 116 % of the nominal concentrations. Because of evaporation, the concentrations slightly increased during the test. The determination of the biological results was based on the nominal concentrations.

The positive control tested in a current reference study, assured that the test conditions were reliable.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. The result of the test is considered valid.

The following results were determined for the test item(species: Daphnia magna).

48h-NOEC = 46 mg/L
48h-LOEC = 100 mg/L

24h-EC₅₀> 100 mg/L
48h-EC₅₀= 213.60 mg/L (extrapolated)

Toxicity to aquatic plants other than algae

This study was performed in order to evaluate the toxicity of tehe test item towards Lemna minor following the OECD Guideline 221 (2006).The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L (nominal concentration). Incubation time was 7 days. The frond number of each replicate was determined at the beginning, at day 2 and 5 during the test and at the end of the experiment. Additionally, the dry mass of 12 representative fronds was determined at the beginning of the experiment. At the end of the experiment the dry mass of each replicate was determined. Growth rate µ and the yield were determined from the frond number and the dry mass at the respective observation times.

Significant inhibition of plant growth was observed at the following concentrations: 10 (only yield dry mass), 22, 46 and 100 mg/L.

At the start and at the end of the test, the content of the test item in the test solutions was determined using photometer. The measured concentrations lay between 94 % and 100 % of the nominal concentrations at the beginning of the test and between 92 % and 103 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentration. The EC₅₀yield frond number was extrapolated by the evaluation program.

The 7d-EC₅₀s of the positive control were determined in a separate reference test and they were in the desired range.

The ErC50 (Lemna minor, 7d) based on frond number for the test item was determined to be greater than 100 mg/L (nominal).

Toxicity to microorganisms

The inhibitory effect of  test item on aerobic waste-water  bacteria was investigated in a respiration test, according to the OECD Guideline 209 (1984).

The IC50 (3 h) was determined to be >100 mg/L nominal conc.

 

JUSTIFICATION FOR CLASSIFICATION OR NON-CLASSIFICATION

According to the CLP Regulation (EC) no. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment when the following criteria are met:

A) Acute (short-term) aquatic hazard Category Acute 1: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/l.

B) Long-term aquatic hazard (iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 1: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/L

Category Chronic 2: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants)> 1 to ≤10 mg/L

Category Chronic 3: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 10 to ≤ 100 mg/L.

The substance is not rapidly degradable and the available acute toxicity test to Oncorhynchus mykis, Daphnia magna and Lemna minor fixed effect levels that do not meet these classification criteria. Therefore, the substance is not classified for aquatic toxicity according to the CLP Regulation (EC) no. 1272/2008.