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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable. Guideline study, well documented and with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1988
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: "Proposed Preliminary Testing Method: prolonged toxicity test on Daphnia magna", according to the Federal Environmental Agency .
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrobenzene
EC Number:
202-716-0
EC Name:
Nitrobenzene
Cas Number:
98-95-3
Molecular formula:
C6H5NO2
IUPAC Name:
nitrobenzene
Details on test material:
- Analytical purity: 99 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Sampling method: GC or HPLC, combined with FID ,ECD , FPD or MS

Test solutions

Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution series of the stock solution

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: IRCHA
- Source: Federal Envrionmental Agency
- Feeding during test
- Food type: Algae and TetraMin



Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

pH:
7.45 - 7.80
Dissolved oxygen:
Saturation of oxygen: about 77 - 90 %
Nominal and measured concentrations:
Nominal test concentrations: 1.56, 3.13, 6.25, 12.5, 25, 50, 100 and 200 mg/L

Details on test conditions:
TEST SYSTEM
- Test vessels filled with 250 ml test substance
- Type (delete if not applicable): open
- Renewal rate of test solution (frequency/flow rate): every 3 days
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater (according to DIN)

OTHER TEST CONDITIONS
- Photoperiod: 9:15 (light:dark)

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
2.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Analytical determined value
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Any other information on results incl. tables

Recovery rates: 
12.5 mg/L (nominal): 21 -29 % recovery
25.0 mg/L (nominal): 14 -22 % recovery 
50.0 mg/L (nominal): 10 -26 % recovery

Applicant's summary and conclusion