Benzoic acid, 3-(trifluoromethyl)-, methyl ester

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Test guideline 2

Qualifier:

according to guideline

Guideline:

ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

457-830-5

EC Name:

-

Cas Number:

2557-13-3

Molecular formula:

Hill formula: C9 H7 F3 O2

IUPAC Name:

Methyl, 3-Trifluoromethylbenzoate

Constituent 2

Reference substance name:

607-745-6

EC Number:

607-745-6

IUPAC Name:

607-745-6

Details on test material:

- Name of test material (as cited in study report): Methyl, 3-Trifluoromethyl Benzoate
- Substance type: mono costituent
- Physical state: liquid
- Analytical purity: 99.77 %
- Lot/batch No.: 250037
- Expiration date of the lot/batch: March 10, 2009
- Stability under test conditions: stable
- Storage condition of test material: room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): Different concentrations of the test item were mixed homogeneously into the soil which was filled in glass vessels before the earthworms were introduced on top of the soil
- Controls: deionised water

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Bred by IBACON in a breeding medium of cattle manure, peat, sand and straw, mainly fed with cattle manure, stored at room temperature.
- Age: ca. 11 to 12 months, with clitellium
- Weight : 300 to 600 mg
- Acclimatisation: 1 day, in artificial soil, under test conditions

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Total exposure duration:

14 d

Test conditions

Test temperature:

Acclimatisation and exposure: 19°C to 21°C

pH:

pH range at experimental start: 5.6 to 6.0
pH range at experimental end: 5.6 to 5.9

Details on test conditions:

TEST SYSTEM
- Test container /Amount of soil :
Normal glass bottling jars (1 L), loosely covered by glass-lids to enable exchange of air and to minimise evaporation, filled with approximately 500 g (dry weight equivalent) artificial soil.
- replicates : 4 per treatment group
- individuals: 10 per test unit, i.e. 40 individuals per treatment group

SOURCE AND PROPERTIES OF SUBSTRATE (artificial soil):
Based on OECD 207 but with reduced organic matter content:
• 5.0% Sphagnum-peat, air-dried and finely ground (2 mm); (Floragard, Vertriebs GmbH für Gartenbau, 26138 Oldenburg, Germany)
• 20% Kaolin clay (Erbslöh, 65558 Lohrheim, Germany)
• approximately 0.2% chalk (CaCO3) added to adjust pH to 6.0 ± 0.5 (Merck, 64293 Darmstadt, Germany)
• approximately 74.8% fine quartz-sand (F34) containing more than 50% by mass of particle size 0.05 mm to 0.2 mm; (Quarzwerke Frechen, Postfach 1780, 50207 Frechen, Germany)

OTHER TEST CONDITIONS
- Light intensity: 400 to 790 lux

Reference substance (positive control):

yes

Remarks:

2-chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

31.25 mg/kg soil dw

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LOEC

Effect conc.:

62.5 mg/kg soil dw

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

168.4 mg/kg soil dw

Any other information on results incl. tables

Effect of Methyl, 3-Trifluoromethyl Benzoate on Earthworm (Eisenia fetida) mortality and biomass (14 d)

Test Species	Eisenia fetida
Exposure	artificial soil
14 d NOEC	31.25 mg/kg dry weight soil
14 d LOEC	62.5 mg/kg dry weight soil
14 d LC50	168.4 mg/kg dry weight soil
	126.0 to 244.0 mg/kgdry weight soil
Treatment Group	Control	Methyl, 3-Trifluoromethyl Benzoate [mg/kg soil dry weight]
	deionised water	31.25	62.5	125	250	500
Mortality after 14 days [%]	0	0	0	0	100	100
Significance (Fisher’s exact test,a= 0.05)	-	n.s.	n.s.	n.s.	*	*
Body weight change after 14 days [%]	-10.4	-13.5	-15.7	-18.5	-	-
Significance (Dunnett-test,a= 0.05)	-	n.s.	*	*	-	-

- not applicable
n.s. = No statistically significant differences compared to the control            * = statistically significantly different to the control

Applicant's summary and conclusion

Conclusions:

In a 14-day acute toxicity study with Methyl, 3-Trifluoromethyl Benzoate to earthworms (Eisenia fetida) the 14-day LC50 was determined to be 168.4 test item/kg soil (95% confidence limits: 126.0 mg/kg to 244.0 mg test item/kg soil.
The No Observed Effect Concentration (NOEC) related to mortality was determined to be 31.25 mg Methyl, 3-Trifluoromethyl Benzoate/kg soil. The Lowest Observed Effect Concentration (LOEC) was determined to be 62.5 mg Methyl, 3-Trifluoromethyl Benzoate/kg soil.