Diammonium peroxodisulphate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Acute bacteria Cell Multiplication Inhibition Test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-07-19 to 1988-08-18

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

1. There was no information on the pH during the test, the test medium used differed slightly from the medium described in DIN 38412 Teil 8 (1991). 2. The growth factor could not be deduced from the report (DIN 38 412 Teil 8: 100 after 18 h). The positive control was reported to fall within the acceptable range (EC10 20611 mg/L; literature EC10 6600 mg/L). The study reliability was lowered, because it cannot be excluded that the growth factor was sub-optimal (DIN 38412 part 8).

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: NEN 6509, Water- Determination of the impact of toxic substances on the growth of a pure culture of bacteria, June 1980

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: NPR 6508, Water- Necessities, method and recipes for the breeding of Pseudomonas fluorescent bacteria, November 1979

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Analytical monitoring:

not specified

Details on sampling:

NA

Vehicle:

no

Details on test solutions:

A fresh test substance solution of 100 mg APS/l in sterile Milli-Q water was prepared. Carefully, the pH of the test substances was adjusted to 7.0 with 0.01M NaOH solution.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Stock cultures of Pseudomonas putida, obtained from RIVM, Bilthoven, The Netherlands, were kept on the nutrient used for stock and preliminary cultures in agar plant tubes. New stock cultures were started at intervals of 1 week. The inoculated stock cultures were incubated at 25°C.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

18 h

Remarks on exposure duration:

at 25 °C

Post exposure observation period:

NA

Hardness:

NA

Test temperature:

25°C

pH:

pH 7

Dissolved oxygen:

NA

Salinity:

NA

Nominal and measured concentrations:

0.6 x 10 E-5 mg to 100 mg/L

Details on test conditions:

NA

Reference substance (positive control):

no

Duration:

18 h

Dose descriptor:

EC10

Effect conc.:

36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: multiplication of bacterial cells

Details on results:

NA

Results with reference substance (positive control):

no positive control

Reported statistics and error estimates:

NA

no remarks

Validity criteria fulfilled:

yes

Conclusions:

Diammonium persulfate was tested in a toxicity to microorganisms study. The determined EC10 (18 h) was 36 mg/L.

Executive summary:

Diammonium persulfate was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to study guidelines NPR 6508 and NEN 6509. Cultures of the bacteria were exposed to concentrations ranging from 6.00E-06 mg to 100 mg/L. An EC10 was determined at 36 mg/L.

Description of key information

The toxicity of microorganisms to diammonium persulfate was tested in a study according to guidelines NEN 6509 and NPR 6508. The study revealed an EC10 (18 h) of 36 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

36 mg/L

Additional information

In the key study the toxicity of diammonium persulfate to the gram negative bacterium Pseudomonas putida was determined in a growth inhibition test according to guidelines NEN 6509 and NPR 6508. Bacteria were exposed in a static test to concentrations of diammonium persulfate up to 100 mg/L. The study revealed an EC10 (18 h) of 36 mg/L.