1,3-bis(1-isocyanato-1-methylethyl)benzene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Mar. 01, 1993 to Mar. 05, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Samples were removed from each treatment on Days 0 and 4 (or at termination of replicate) and analyzed for Dissolved Organic Carbon (DOC) content

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Differential loading: 0.65 g/L
- Controls: Yes
- Individual treatments were prepared by adding the appropriate amount of test material to laboratory dilution water. The treatments were slowly mixed (<10% vortex) on a magnetic striplate with a teflon coated stirbar for approximately 48 hours. During mixing, clear globules of the test material were observed throughout the water column. An oily surface slick was also observed in all treatments. After the mixing period, the water column appeared clear with clear globules of test material on the bottom. The Water Accomodated Fraction (WAF) of each treatment solution was siphoned from the middle portion of the battery jar using a glass siphon and silicone tubing and divided into 2 replicate chambers. Test chambers were covered with glass to minimize evaporation and/or volatilization.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: Bluegill sunfish
- Source: Osage Cat Fisheries, Inc., Osage Beach, MO 65065
- Age at study initiation: ~ 29 wk
- Length at study initiation: 2.6 cm (SD 0.2)
- Weight at study initiation: 0.209 g (SD 0.081)
- Feeding during test: No

ACCLIMATION
- Acclimation period: At least 12 d
- Acclimation conditions:
- Food type: Rangen Salmon Starter and/or ≤ 48-h old brine shrimp nauplii

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

No data

Test temperature:

22.3 °C

pH:

7.0- 7.8

Dissolved oxygen:

5.7-8.0 mg/L

Salinity:

No data

Nominal and measured concentrations:

Nominal:
- Range finding test: 0, 5, 10, 50, 100 and 1,000 mg/L
- Definitive test: 0, 60, 120, 250, 500, 1,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 8.5 L glass tank/3.2 L
- No. of organisms per vessel: Ten
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two
- Biomass loading rate: 0.65 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water
- Dissolved oxygen: 8.5-8.7 mg/L
- Alkalinity: 27-28 mg/L
- Conductivity: 170 µ mhos


OTHER TEST CONDITIONS
- Photoperiod: 15 h light/9 h dark
- Light intensity: 61.74-63.61 foot candles


- Range finding study
- Test concentrations: 5, 10, 50, 100 and 1,000 mg/L
- Results used to determine the conditions for the definitive study: Mortality (10 %) at 1,000 mg/L after 48 h. Since no other mortality occurred during the 96-h period, it is believed that the mortalities were caused by contamination of the test chamber.

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 65.88 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Sublethal observations / clinical signs:

Table 1: Analytical results

Nominal chemical conc (mg/L)	DOC (ppm)		Measured chemical concentration*
	Day 0	Day 4	Day 0	Day 4
Control	5.408	3.878	-	-
60	10.07	12.19	6.772	12.07
120	41.27	42.90	52.09	56.68
250	24.25	23.57	27.37	28.60
500	45.51	35.63	58.25	46.12
1,000	45.46	53.54	58.18	73.58

*Treatment levels were converted from nominal to measured values as follows: (Treatment DOC value - control DOC value)/% carbon of the test material

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 96 h LC50 of the test material to bluegill sunfish was determined to be > 65.88 mg/L based on exposure to a Water Accommodated Fraction (WAF) of the test material.

Executive summary:

A study was performed to evaluate the acute toxicity of the test substance to bluegill sunfish (Lepomis macrochirus) under static conditions. The study was conducted according to OECD Guideline 203, in compliance with GLP. Twenty fish per dose were exposed to a Water Accommodated Fraction (WAF) of the test substance at 0, 60, 120, 250, 500, 1000 mg/L (nominal). Test substance concentrations (as Dissolved Organic Carbon (DOC)) were analysed on Days 0 and 4. Mortality and behavioural effects were recorded at 24, 48, 72 and 96 h. Total mortality was observed in one replicate of the 1000 mg/L treatment. Since no other mortality occurred during the 96 h period, it is believed that the mortality was caused by contamination of the test chamber. Under the study conditions, the 96 h LC50 of the test substance to bluegill sunfish was determined to be > 65.88 mg/L (measured) based on exposure via a WAF (Targia, 1993).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Jul. 07, 1986 to Jul. 11, 1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle: Acetone
- The definitive test concentration was obtained by transfering appropriate aliquots of a working standard directly into the test chamber. No corrections were made for sample purity (98-99%).

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Source: ABC in-house culture
- Age at study initiation: ~ 29 wk
- Length at study initiation: 19 ± 2.1mm
- Weight at study initiation: 0.099 ± 0.037 g
- Feeding during test: No feeding during test

ACCLIMATION
- Acclimation period: At least 14 d
- Acclimation conditions (same as test or not):
- Food type: Standard commercial fish food supplemented with brine shrimp nauplii
- Frequency: Daily until 48-96 h prior to testing

- Loading biomass: 0.066 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

40-45 mg/L as CaCO3

Test temperature:

22 ± 1 °C

pH:

6.9-7.7

Dissolved oxygen:

4.9-9.2 mg/L

Salinity:

No data

Nominal and measured concentrations:

Nominal concentrations:
- Range finding study: 0, 0.01, 0.1, 1, 10 and 100 mg/L
- Definitive study: 0, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water
- Dissolved oxygen: 9.2- 10.1 mg/L
- Total alkalinity: 325- 375 ppm as CaCO3
- pH: 7.8- 8.3
- Pesticides: Less than minimum detectable limits
- Conductivity: 700 µ mhos/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 h day light



- Range finding study
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

not required

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

0.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 0.56-1.0

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 0.32-1.0

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 0.32-1.0

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Mortality: 10-20 % and 100 % mortality in 0.56 and 1.0 mg/L treatment groups
- Abnormal responses: Surfacing, loss of equilibrium and/or quiescence at 0.56 and 1.0 mg/L

Reported statistics and error estimates:

Statistical analysis was performed using a computerized LC50 program

Sublethal observations / clinical signs:

The 0.32, 0.56 and 1.0 mg/L solutions had a light surface film after stirring. The surface film was no longer visible in any test chamber after 24 hours of testing.

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 96 h LC50 of the test material to fathead minnow was determined to be 0.67 mg/L (0.32-1.0 mg/L). The NOEC was estimated to be 0.32 mg/L, based on lack of mortality and abnormal effects at this dose.

Executive summary:

A study was performed to evaluate the acute toxicity of the test substance to fathead minnow (Pimephales promelas) under static conditions. The study was conducted according to a method equivalent or similar to OECD Guideline 203, in compliance with GLP. Ten fish per dose were exposed at 0, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L. Mortality and behavioural effects were recorded at 24, 48, 72 and 96 h. Mortality, surfacing, loss of equilibrium and/or quiescence were observed during the 96 h exposure period at 0.56 and 1.0 mg/L. The 0.32, 0.56 and 1.0 mg/L solutions had a light surface film after stirring. The surface film was no longer visible in any test chamber after 24 h of testing. Under the study conditions, the 96 h LC50 of the test substance to fathead minnow was determined to be 0.67 mg/L (0.32-1.0 mg/L). The NOEC was estimated to be 0.32 mg/L, based on lack of mortality and abnormal effects at this dose (Swigert, 1986).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.67 mg/L

Additional information

A study was performed to evaluate the acute toxicity of the test substance to bluegill sunfish (Lepomis macrochirus) under static conditions. The study was conducted according to OECD Guideline 203, in compliance with GLP. Twenty fish per dose were exposed to a Water Accommodated Fraction (WAF) of the test substance at 0, 60, 120, 250, 500, 1000 mg/L (nominal). Test substance concentrations (as Dissolved Organic Carbon (DOC)) were analysed on Days 0 and 4. Mortality and behavioural effects were recorded at 24, 48, 72 and 96 h. Total mortality was observed in one replicate of the 1000 mg/L treatment. Since no other mortality occurred during the 96 h period, it is believed that the mortality was caused by contamination of the test chamber. Under the study conditions, the 96 h LC50 of the test substance to bluegill sunfish was determined to be > 65.88 mg/L (measured) based on exposure via a WAF (Targia, 1993).

A study was performed to evaluate the acute toxicity of the test substance to fathead minnow (Pimephales promelas) under static conditions. The study was conducted according to a method equivalent or similar to OECD Guideline 203, in compliance with GLP. Ten fish per dose were exposed at 0, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L. Mortality and behavioural effects were recorded at 24, 48, 72 and 96 h. Mortality, surfacing, loss of equilibrium and/or quiescence were observed during the 96 h exposure period at 0.56 and 1.0 mg/L. The 0.32, 0.56 and 1.0 mg/L solutions had a light surface film after stirring. The surface film was no longer visible in any test chamber after 24 h of testing. Under the study conditions, the 96 h LC50 of the test substance to fathead minnow was determined to be 0.67 mg/L (0.32-1.0 mg/L). The NOEC was estimated to be 0.32 mg/L, based on lack of mortality and abnormal effects at this dose (Swigert, 1986).