2-methyl-2H-isothiazol-3-one

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not stated in report

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted according to guideline/s and GLP but the report contains sufficient data for interpretation of study results.

Qualifier:

no guideline available

Principles of method if other than guideline:

Groups of fish were exposed to 14C RH-886 Technica which consists of CMIT and MIT for up to 56 days and subsequently to fresh water for up to 14 days. Water and fish samples were radioassayed at selected intervals.

GLP compliance:

no

Remarks:

prior to GLP implementation

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Radiolabelling:

yes

Vehicle:

no

Test organisms (species):

Lepomis macrochirus

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Total depuration duration:

14 d

Nominal and measured concentrations:

The concentrations to which the fish were exposed were nominally 0.02, 0.12, and 0.80 ppm of RH-886 for Formulation 1 and 0.12 ppm of RH-886 for Formulation 2.

Reference substance (positive control):

not specified

Type:

BCF

Value:

5.75 dimensionless

Basis:

other: carcass

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.12 ppm

Type:

BCF

Value:

48.1 dimensionless

Basis:

other: viscera

Remarks on result:

other: Conc.in environment / dose:0.12 ppm

Elimination:

yes

Parameter:

DT50

Depuration time (DT):

5 d

Metabolites:

No data

Results with reference substance (positive control):

Not applicable

Details on results:

The steady-state residue values found in the carcass and viscera of fish were determined as follows:

Formulation 1/0.02 ppm of 14C RH-886: 0.609 ppm in carcass and 4.08 ppm in viscera

Formulation 1/0.12 ppm of 14C RH-886: 3.19 ppm in carcass and 18.8 ppm in viscera

Formulation 1/0.8 ppm of 14C RH-886: 17.8 ppm in carcass and 240 ppm in viscera

Formulation 2/0.12 ppm of 14C RH-886 : 0.575 ppm in carcass and 4.81 ppm in viscera

Following exposure of fish to 14C RH-886 labelled on the RH-573 component, the resulting BCF of 14C residues was 5.75 in carcass and 48.1 in viscera.

Elimination of 50% of 14C residues took place in less than 7 days in all cases.

Validity criteria fulfilled:

yes

Conclusions:

Following exposure of fish to 14C RH-886 labelled on the RH-573 component, the resulting BCF of 14C residues was 5.75 in carcass and 48.1 in viscera.

Elimination of 50% of 14C residues took place in less than 7 days in all cases.

Executive summary:

The bioaccumulation potential of ¹⁴C labelled MIT was examined in Lepomis macrochirus (bluegill). Fish were exposed to 0.12 ppm MIT under flow through conditions for up to 56 days. Following exposure to the test material, some fish were exposed to fresh water for up to 14 days. After 56 days exposure, 0.575 ppm labelled material was found in the carcass and 4.81 ppm labelled material was found in viscera. Following exposure of fish to ¹⁴C RH-886 labelled on the RH-573 component, the resulting BCF of ¹⁴C residues was 5.75 in carcass and 48.1 in viscera. Elimination of 50% of ¹⁴C residues took place in less than 7 days in all cases.

Description of key information

The bioaccumulation potential of 14C labelled MIT was examined in Lepomis macrochirus (bluegill). Fish were exposed to 0.12 ppm MIT under flow through conditions for up to 56 days. Following exposure to the test material, some fish were exposed to fresh water for up to 14 days. After 56 days exposure, 0.575 ppm labelled material was found in the carcass and 4.81 ppm labelled material was found in viscera. Following exposure of fish to 14C RH-886 labelled on the RH-573 component, the resulting BCF of 14C residues was 5.75 in carcass and 48.1 in viscera. Elimination of 50% of 14C residues took place in less than 7 days in all cases.