2-benzylideneheptanal

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from J-check authoritative database

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute toxic effect on the Daphnia species was observed by using the chemical alpha-Amyl cinnamaldehyde.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): alpha-Amyl cinnamaldehyde
- Molecular formula (if other than submission substance): C14H18O
- Molecular weight (if other than submission substance): 202.2952mg/l
- Smiles notation (if other than submission substance): c1(\C=C(\CCCCC)C=O)ccccc1
- InChl (if other than submission substance): 1S/C14H18O/c1-2-3-5-10-14(12-15)11-13-8-6-4-7-9-13/h4,6-9,11-12H,2-3,5,10H2,1H3/b14-11-
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: No data
- Analytical purity: No data

Analytical monitoring:

not specified

Details on sampling:

No data

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

Daphnia sp.

Details on test organisms:

No data

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

No data

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.28 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No data

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

not specified

Conclusions:

Based on the immobilization of the test organism daphnia species by the alpha-Amyl cinnamaldehyde, the EC50 is 0.28mg/l.

Executive summary:

Acute toxic effect on the Daphnia species was observed by using the chemical alpha-Amyl cinnamaldehyde, with the 48hrs total exposure period.

 

Based on the 0.28mg/l EC50 value, it can be concluded that the test chemicalalpha-Amyl cinnamaldehyde can be considered as toxic to the aquatic organism daphnia at environment revelent concentration and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.    

 

Description of key information

Based on the experimental key study (J-check authoritative databases, 2016) acute toxic effect on the Daphnia species was observed by using the chemical alpha-Amyl cinnamaldehyde(122 -40 -7) 2 -benzylideneheptanal, with the 48hrs total exposure period. Based on the 0.28mg/l EC50 value, it can be concluded that the test chemical alpha-Amyl cinnamaldehyde can be considered as toxic to the aquatic organism daphnia at environment revelent concentration and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.   

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.28 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical have been reviewed to determine the toxic nature of alpha-Amyl cinnamaldehyde (122-40-7), IUPAC name 2 -benzylideneheptanal on the growth of acquatic invertebrates.The studies are as mentioned below:

Based on the experimental key study (J-check authoritative databases, 2016) acute toxic effect on the Daphnia species was observed by using the chemical alpha-Amyl cinnamaldehyde, with the 48hrs total exposure period. Based on the 0.28mg/l EC50 value, it can be concluded that the test chemical alpha-Amyl cinnamaldehyde can be considered as toxic to the aquatic organism daphnia at environment revelent concentration and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.   

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 0.946mg/l for CAS name 122 -40 -7 in 48 hrs. Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

 

Similarly in the supporting study (HPVIS, 2017) acute toxic effect on the Daphnia species was carried out by using the chemical alpha-Amyl cinnamaldehyde for 48 hrs. Static method was use for the study with different concentrations. At various concentration EC value and NOEC obtain at 48 hours is EC0=0.4 mg/L and 48 hr. EC50=1.1 mg/L; EC100=2.8 mg/l and NOEC is 0.4 mg/l. The number of Daphnis exhibiting immobilization at 48hours for duplicate runs at each mean measured concentration was also observed. In control and 0.1mg/l no changes were obtain. But in 0.7 mg/l =4/40 & 5/40; 1.4 mg/L= 25/40 & 31/40; and 2.8 mg/L= 40/40 & 32/40. Based on the 1.1mg/l EC50 value, it can be concluded that the test chemical alpha-Amyl cinnamaldehyde can be considered as toxic to the aquatic organism daphnia at environment revelent concentration and thus can be considered to be classified in aquatic chronic category 2 as per the classification criteria.

 

In second supporting study (Inventory Multitiered Assessment and Prioritisation, NICNAS, 2017) acute toxic effect on the Daphnia magna was observed by using the chemical alpha-Amyl cinnamaldehyde. Based on the 1.1mg/l EC50 value, it can be concluded that the test chemical alpha-Amyl cinnamaldehyde can be considered as toxic to the aquatic organism daphnia at environment revelent concentration and thus can be considered to be classified in chronic category 2 as per the classification criteria.

 

Although 2 study from secondary sources gives the EC50 value greater than 1, but the data from the J-check authoritative databases (K2) which was the key study, EC50 was 0.28mg/l and performed as per the OECD guideline. Thus we consider that the chemical alpha-Amyl cinnamaldehyde to be toxic to aquatic environment and can be considered to be classified in acute category 1as per the CLP classification criteria.