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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not GLP.

Data source

Reference
Reference Type:
publication
Title:
The Acute Toxicity of 102 Pesticides and Miscellaneous Substances to Fish
Author:
Tooby TE, Hursey PA, Alabaster JS
Year:
1975
Bibliographic source:
Chemistry and Industry, 21: 523-526

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Alabaster and Abram 1965a
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Warfarin
EC Number:
201-377-6
EC Name:
Warfarin
Cas Number:
81-81-2
Molecular formula:
C19H16O4
IUPAC Name:
4-hydroxy-3-(3-oxo-1-phenylbutyl)-2H-chromen-2-one
Details on test material:
- Name of test material (as cited in study report): Warfarin.
- Analytical purity: Commercial formulation, 100%.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Rasbora heteromorpha
Details on test organisms:
TEST ORGANISM
- Common name: Harlequin fish.
- Strain: Duncker.
- Length at study initiation (length definition, mean, range and SD): 1-3 cm.


ACCLIMATION
- Acclimation period: At least a week.
- Acclimation conditions (same as test or not): Yes.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None.

Test conditions

Hardness:
20 mg/L as CaCO3.
Test temperature:
20° Celcius.
pH:
8.1 SU.
Dissolved oxygen:
Not specified.
Salinity:
Not applicable.
Nominal and measured concentrations:
Not specified.
Details on test conditions:
TEST SYSTEM
- Test vessel: Not specified.
- Aeration: No.
- Renewal rate of test solution (frequency/flow rate): Daily.


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Not specified.


OTHER TEST CONDITIONS
- Adjustment of pH: No.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48
Dose descriptor:
LC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Reported statistics and error estimates:
LC50s calculated after Litchfield and Wilcoxon, 1949.

Applicant's summary and conclusion

Conclusions:
The 96 hour LC50 for Warfarin to Harlequin fish is 12 mg/L.