Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1997

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): RA 3643
- Substance type: white powder
- Physical state: solid
- Lot/batch No.: 87/96
- Storage condition of test material: refrigerator temperature.
The test substance was stated to be insoluble in water, and its purity was stated to be > 99%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
For the limit test 270 mg was dissolved in 10 ml of DMSO, from which 100 mcl was dispersed in one litre of medium, so as to yield a test substance concentration of 2.7 mg/l

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The fresh water green alga Selenastrum capricornutum was supplied by the 'American Type Culture Collection', c/o Sales Department, USA.

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
68 h

Test conditions

pH:
The pH was measured at the start and after 68h in selected cultures.
The pH was found to have remained stable during the test (pH 8.2-8.4)
Reference substance (positive control):
yes
Remarks:
K2Cr2O7 and/or 3.5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
68 h
Dose descriptor:
NOEC
Effect conc.:
>= 2.7 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Duration:
68 h
Dose descriptor:
NOEC
Effect conc.:
>= 2.7 mg/L
Nominal / measured:
nominal
Basis for effect:
biomass
Duration:
68 h
Dose descriptor:
EC50
Effect conc.:
> 2.7 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Duration:
68 h
Dose descriptor:
EC50
Effect conc.:
> 2.7 mg/L
Nominal / measured:
nominal
Basis for effect:
biomass

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No inhibition of any significance was observed with the RA3643. Of the three parameters involved, the effect on the vialibility of the inoculum was considered less reliable and less relevant than the other parameters.