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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Analytical determinations and new study director
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. QA statement)
Remarks:
Swiss GLP
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR50
- Expiration date of the lot/batch: Sep 2004

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: > 80 g/l (in water)
Analytical monitoring:
yes
Details on sampling:
During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
Details on test solutions:
Stock solution 300.0 mg test substance was mixed with about 200 ml test medium in a beaker, then homogenised by ultrasonic treatment for 2 minutes and filled up to 3000 ml with test medium.
Test concentrations: Due to the results of a pre-test the nominal test concentrations were chosen as 100 mg test substance/l.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
The test organism was the species zebrafish (Brachydanio rerio) supplied by Charles River Aquatics, Sorneren, Netherlands. The quality of the fish was checked with an acclimatization time of 89 days in dechlorinated tap water.
For each concentration, 7 fish were used. The fish were not fed during the test.
Weight of fish : 0.62 g/fish (average)
Length of fish : 26 mm (average)
Treatment : none
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
142 mg CaCO3/L
Test temperature:
21.1 - 21.4 °C
pH:
7.9 - 8.4
Dissolved oxygen:
95 - 98%
Nominal and measured concentrations:
During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
Neither mortality nor symptoms regards swimming behaviour, loss of equilibrium or repsiratory function nor pigmentation was observed in fist exposed to FAT40571/A.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Due to lack of effects at limi concentration no statistics was applied.
Sublethal observations / clinical signs:

Due to the results of a pre-test the nominal concentration of FAT 40571/A for the main test was selected as 100 mg test substance/1. Additionally, a blank control without test substance was tested.

During the test the actual test concentration in the analysed test media was in the range of 96.3 - 98.1 % of the nominal values. Therefore, the EC-values were determined from the nominal test concentration.

The LC50 value after 96 hours was determined to be >100 mg/l.

Validity criteria fulfilled:
yes
Conclusions:
The LC50 and NOEC of FAT 40571/A after 96 h (zebrafish) was determined to be >100 mg/l
Executive summary:

Acute toxicity of FAT 40571/A for zebrafish was carried out according to OECD 203 and EU C1 guideline to determine the LC50 (concentration at which 50% of the fish population died).

In total 7 fish at each test concentration (100 mg test substance / 1 nominal) used in this static test at 21.1 - 21.4°C and tested for 96 h. Analysis was carried out using Spectophotometry.

During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 9 8 . 1% of the nominal values. The results are related to the nominal values of the test substance concentrations.

 

The LC50 value after 96 hours was determined to be >100 mg/1. So, in conclusion, the ecotoxicological classification of FAT 40571/A is not toxic to zebrafish after an exposure period of 96 h.

Description of key information

The LC50 and NOEC of Reactive Brown 49 after 96 h (zebrafish) was determined to be >100 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Acute toxicity of Reactive Brown 49 for zebrafish was carried out according to OECD 203 and EU C1 guideline to determine the LC50 (concentration at which 50% of the fish population died).


In total 7 fish at each test concentration (100 mg test substance / 1 nominal) used in this static test at 21.1 - 21.4°C and tested for 96 h. Analysis was carried out using Spectophotometry.


During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.


 


The LC50value after 96 hours was determined to be >100 mg/l. So, in conclusion, the ecotoxicological classification of Reactive Brown 49 is not toxic to zebrafish after an exposure period of 96 h.