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reaction mass of: 7-amino-3,8-bis-[4-(2-sulfoxyethylsulfonyl)phenylazo]-4- hydroxynaphthalene-2-sulfonic acid, Na/K salt; 7-amino-3-[4-(2-sulfoxyethylsulfonyl)phenylazo]-4-hydroxy-8-[4-(2-sulfoxyethylsulfonyl)-2- sulfophenylazo]naphthalene-2-sulfonic acid, Na/K salt; 7-amino-8-[4-(2-sulfoxyethylsulfonyl)-phenylazo]-4-hydroxy-3-[4-(2-sulfoxyethylsulfonyl)- 2-sulfophenylazo]naphthalene-2-sulfonic acid, Na/K salt; 7-amino-3,8-bis-[4-(2-sulfoxyethylsulfonyl)-2-sulfophenylazo]-4-hydroxynaphthalene-2- sulfonic acid, Na/K salt
EC number: 429-070-4 | CAS number: 214362-06-8 SCARLET DER 8107
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 17,1998 to February 9,1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Switzerland GLP
- Type of method:
- batch equilibrium method
- Media:
- soil
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR50
- Expiration date of the lot/batch: September 1,2004
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature
- Stability under test conditions: Stable for 24 hours
- Solubility of the test substance in the solvent/vehicle: > 80 g/l (at 20 °C) - Radiolabelling:
- no
- Test temperature:
- 20 ± 1 °C.
- Details on study design: HPLC method:
- The following HPLC conditions were developed in a preliminary test. The aqueous phases of all tubes were analysed for parent concentration. Standard solutions were analysed before a series; of samples at each sampling. For each series a linear regression curve was calculated using the standard solution concentrations and the corresponding peak areas. With this linear regression curve the concentrations in the aqueous samples were calculated.
Operating Conditions
Column: 12.5 cm x 4.0 mm, 5 urn Supersphere 100 C18
Flow: 1 ml/minute
Mobile phase:
Solvent A: 1 g TBAPC + 100 ml acetonitrile + 50 ml buffer solution (pH 7) add 1 litre distilled water
Solvent B: 1 g TBAPC + 250 ml distilled water + 50 ml buffer solution (pH 7) add 1 litre acetonitrile
TBAPC: Tetrabutylammoniumperchlorat
Buffer: Merck Titrisol-buffer, Art. no. 9887
Gradient:
Time [min.] 0 20 21 25
%A 95 20 95 95
%B 5 80 5 5
Injection: 50 µl
Retention time: approx. 17 to 18 min. - Analytical monitoring:
- yes
- Details on matrix:
- Three soils: Speyer (loamy sand) Sisseln (sandy loam) and Les Barges (silt loam).
- Type:
- Koc
- Remarks:
- Mean of three soil types
- Value:
- 506 other: ml/g
- Temp.:
- 20 °C
- % Org. carbon:
- 2.55
- Remarks on result:
- other: mean value of the three type of soils (666, 433 and 420 ml/g)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance can be regarded as little mobile in all three soils (loamy sand, sandy loam and silt loam). The average KOC found was 506 ml/g.
- Executive summary:
The adsorption and desorption behaviour of FAT 40'571/A was determined in a screening test by the batch equilibrium method using three soils: Speyer (loamy sand) Sissein (sandy loam) and Les Barges (silt loam). The study was carried out according to OECD 106 guideline. The soils chosen represented a range of organic carbon content, pH, cation exchange capacity and clay content.
A preliminary test was performed to establish a suitable analytical procedure. CaCI2solutions were analysed for parent by High Performance Liquid Chromatography (HPLC). The substance could be analysed in a concentration range of 0.5 mg/l to 9.0 mg/l. The air-dried soils were conditioned with 0.01 mol CaCI2per litre solution before the test.
For the screening test, 5 g dried soil equivalents were treated with 25 ml of a 0.01 mol CaCI2per litre solution containing the test item. This adsorption was performed in duplicate at a concentration of 4.806 mg FAT 40'571/A per litre. The amount of FAT 40'571/A adsorbed to soil was 75.3% for soil Speyer, 58.0% for soil Sissein and 76.2% for soil Les Barges.
After the adsorption, two desorption steps were performed. Therefore, 20-23 ml of untreated 0.01 mol CaCI2per litre solution was added to the soil sample and the mixture was shaken for 16 hours. From soils Speyer, Sissein and Les Barges less than 22.5%, 31.6% and 16.8% of the adsorbed FAT 40'571/A was desorbed. Corresponding in the three soils more than 77.4%, 69.8% and 83.2% of the adsorbed test item remained adsorbed. The adsorption coefficient K' was calculated as follows:
K'oc (ml/g) Organic Carbon (g/100g dry soil) K'ow (ml/g)
- Spayer (loamy sand) 666 2.29 386
- Sisseln (sandy loam) 433 1.57 251
- Les Barges (silt loam) 420 3.80 244The K'oc values show that FAT 40'571/A is little mobile in all three soils.
Reference
Adsorption
The adsorption test was performed with an application solution (0.01 mol CaCI2) with 8.21 mg FAT 40'571/A per litre. The volumes of the CaCI2solutions were determined gravimetrically. The total volume (V0) of the aqueous phase is the sum of 15 ml application solutions and the 10 ml CaCI2solution of equilibration of the soil. After 16 h of adsorption, the CaCI2solution was removed for analysis. Two control tubes without soil were treated correspondingly. The quantity of the test item recovered from the controls was determined to be 120.15 mg/l. This corresponded to 97.6% of the application. No relevant
adsorption of FAT 40'571/A on the tube was observed.
Soils Speyer, Sisseln and Les Barges adsorbed 75.3%, 58.0% and 76.2%, respectively, of FAT 40'571/A within 16 h.
Desorption
After 16 hours of adsorption, two desorption steps were performed. From soils Speyer, Sisseln and Les Barges less than 22.5%, 31.6% and 16.8% of the adsorbed FAT 40'571/A was desorbed. The concentrations of FAT 40'571/A desorbed were below the limit of quantification (0.5 mg/l) for the second desorption from all soils and for the first desorption of soil Les Barges. For the calculation of percent desorbed the limit of quantification was used for these concentrations to give an estimation of maximum
desorption.
Correspondingly in the three soils more than 77.4%, 69.8% and 83.2% of the adsorbed amount remained adsorbed. Thus, it can be stated that FAT 40'571/A is strongly adsorbed to the soils tested.
Determination of the Adsorption Coefficient
The adsorption coefficient was calculated taking into account the quantity of FAT 40'571/A adsorbed after 16 h, the soil dry mass and the concentration of FAT 40'571/A remaining in solution after 16 h.
The adsorption coefficients K' for FAT 40'571/A in soils Speyer, Sisseln and Les Barges were 15.24 ml/g, 6.81 ml/g and 15.97 ml/g, respectively.
The adsorption coefficient K' can be related to the organic carbon content (in percent) of the soil resulting in a K'oc value. The K'oc value of FAT 40'571/A in soil Speyer, Sisseln and Les Barges was 666 ml/g, 433 ml/g and 420 ml/g, respectively. The respective K'OM values were calculated as 386 ml/g, 251 ml/g and 244 ml/g.
Description of key information
The Reactive Brown 49 is a little mobile in all three soils.
Key value for chemical safety assessment
- Koc at 20 °C:
- 506
Additional information
The adsorption and desorption behaviour of Reactive Brown 49 was determined in a screening test by the batch equilibrium method using three soils: Speyer (loamy sand) Sissein (sandy loam) and Les Barges (silt loam). The study was carried out according to OECD 106 guideline. The soils chosen represented a range of organic carbon content, pH, cation exchange capacity and clay content.
A preliminary test was performed to establish a suitable analytical procedure. CaCI2solutions were analysed for parent by High Performance Liquid Chromatography (HPLC). The substance could be analysed in a concentration range of 0.5 mg/l to 9.0 mg/l. The air-dried soils were conditioned with 0.01 mol CaCI2per litre solution before the test.
For the screening test, 5 g dried soil equivalents were treated with 25 ml of a 0.01 mol CaCI2per litre solution containing the test item. This adsorption was performed in duplicate at a concentration of 4.806 mg Reactive brown 49 per litre. The amount of Reactive brown adsorbed to soil was 75.3% for soil Speyer, 58.0% for soil Sissein and 76.2% for soil Les Barges.
After the adsorption, two desorption steps were performed. Therefore, 20-23 ml of untreated 0.01 mol CaCI2per litre solution was added to the soil sample and the mixture was shaken for 16 hours. From soils Speyer, Sissein and Les Barges less than 22.5%, 31.6% and 16.8% of the adsorbed Reactive Brown 49 was desorbed. Corresponding in the three soils more than 77.4%, 69.8% and 83.2% of the adsorbed test item remained adsorbed. The adsorption coefficient K' was calculated as follows:
K'oc (ml/g) Organic Carbon (g/100g dry soil) K'ow (ml/g)
- Spayer (loamy sand) 666 2.29 386
- Sisseln (sandy loam) 433 1.57 251
- Les Barges (silt loam) 420 3.80 244
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