trans-1-[4-(4-Propylcyclohexyl)-1-cyclohexen-1-yl]-4- (trifluoromethyl)-benzene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Dec 02, 2015 - Jan 07, 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

HJ/T 155-2004 The Guidelines of Good Laboratory Practices on Chemical Testing, MEP, China.

Justification for type of information:

This study was performed according OECD TG 207. The quality system applied was HJ/T 155-2004 The Guidelines of Good Laboratory Practices on Chemical Testing, MEP, China.

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

no

Remarks:

no OECD GLP

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Acetone

Details on preparation and application of test substrate:

Artificial soil test substrate was prepared as follows:
a) 10 per cent sphagnum peat (pH 6.0, no visible plant remains, finely ground, dried).
b) 20 per cent kaolin clay (kaolinite content preferably above 30 per cent).
c) 70 per cent industrial sand (fine sand should be dominant with 60 per cent of the
particles between 50 and 200 microns).
d) pH is adjusted to 6.0 by addition of calcium carbonate.
The dry constituents were blended in the correct proportions and mixed thoroughly with a
large-scale laboratory mixer.


Test subsance amount was calculated based on dry weight

Test organisms (species):

Eisenia fetida

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia fetida
- Strain: Daiping II
- Source:Jia Liming Earthworm Cultivation Ltd. Tianjin
- Breeding generation: EF A000-20150803-01
- Age at test initiation (mean and range, SD): 2 month


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): no feeding
- Health during acclimation (any mortality observed): no mortality, no other findings

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

19.5 - 20.4 °C

pH:

5.83

Moisture:

33.99% dry soil weight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4



OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 400 - 800 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 0 and 14

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
Pre Test: 10, 100, 500 and 1000 mg/kg dw
Main Test: 0, 1000 mg/kg dw

Nominal and measured concentrations:

0, 1000 mg/kg dw

Reference substance (positive control):

no

Key result

Duration:

14 d

Dose descriptor:

LD50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

7 d

Dose descriptor:

LD50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Reported statistics and error estimates:

Standard statistical methods have been applied for data processing.

Validity criteria fulfilled:

yes

Conclusions:

Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations was higher than 1000 mg/kg dry soil weight.

Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The GLP (China) criteria were met and the methods applied are fully compliant with OECD TG 207.

Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations was higher than 1000 mg/kg dry soil weight.

Description of key information

The informtion for this endpoint study record was obtained from an experimental study. The GLP (China) criteria were met and the methods applied are fully compliant with OECD TG 207. Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw