Trisodium hydrogen [2-[[α-[[3-[[4-chloro-6-[ethyl[3-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulphophenyl]azo]benzyl]azo]-4-sulphobenzoato(6-)]cuprate(4-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Principles of method if other than guideline:

WoE report is based on two toxicity to microorganism studies as-
WoE 2. and WoE 3.

GLP compliance:

not specified

Analytical monitoring:

no

Remarks:

WoE 2: no and WoE 3: no data available

Details on sampling:

WoE 2: - Concentrations: Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l.
WoE 3: no data available

Vehicle:

not specified

Test organisms (species):

activated sludge, domestic

Details on inoculum:

WoE 2: - Source: Test organism activated sludge was obtained from laboratory sewage treatment apparatus (Husman).
WoE 3: no data available

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test temperature:

WoE 2: 21.1°C
WoE 3: no data available

Reference substance (positive control):

yes

Remarks:

WoE 2: 3,5-dichlorophenol was used as a reference substance and WoE 3: no data available

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: WoE 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: WoE 2 and WoE 3

Details on results:

WoE 2: A significant inhibition of the respiration rate was not observed at any concentration.
WoE 3: no data available

Results with reference substance (positive control):

WoE 2: The IC50 value of the reference substance 3,5-dichlorophenol was determined to be within 10-25 mgO2/l.
WoE 3: no data available

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.

Executive summary:

Data available of the read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

Toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test" at 21.1°C temperature. Test organism activated sludge (domestic) was obtained from laboratory sewage treatment apparatus (Husman). Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l. The test concentrations were not verified analytically. 3,5 -dichlorophenol was used as a reference substance. Oxygen-consumption was measured with an electrode system. The IC50 value of the reference substance 3,5 -dichlorophenol was determined to be within 10 -25 mgO2/l. No significant inhibition of the respiration rate was observed at any concentration. Thus, on the basis of effect on respiration rate of the test organism activated sludge, the EC0 and EC50 value was determined to be 100 and > 100 mg/l.

For the test chemical, toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test". Activated sludge (domestic) was used as a test organism. On the basis of effect on respiration rate of the test organism, the EC50 value was determined to be > 100 mg/l.

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.

Description of key information

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.

Additional information

Data available of the read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

 

Toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test" at 21.1°C temperature. Test organism activated sludge (domestic) was obtained from laboratory sewage treatment apparatus (Husman). Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l. The test concentrations were not verified analytically. 3,5 -dichlorophenol was used as a reference substance. Oxygen-consumption was measured with an electrode system. The IC50 value of the reference substance 3,5 -dichlorophenol was determined to be within 10 -25 mgO2/l. No significant inhibition of the respiration rate was observed at any concentration. Thus, on the basis of effect on respiration rate of the test organism activated sludge, the EC0 and EC50 value was determined to be 100 and > 100 mg/l.

 

For the test chemical, toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test". Activated sludge (domestic) was used as a test organism. On the basis of effect on respiration rate of the test organism, the EC50 value was determined to be > 100 mg/l.

 

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.