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EC number: 247-475-2 | CAS number: 26134-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-01-17 to 2017-01-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 13th April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- adopted 30th May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Expiration date of the batch: 2017-03-11
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature (20 ± 5 °C); Keep under inert gas
OTHER SPECIFICS: fine powder, white to grey - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 4.6, 10, 22, 46, 100 mg/L
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin
- Age of parental stock: between 0 and 24 hours
- Feeding during test: No
ACCLIMATION
- Acclimation conditions (same as test or not): same as test conditions
- Type of food: unicellular green algae (Desmodesmus subspicatus)
- Health during acclimation (any mortality observed): No. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 19.2 - 20.9 °C
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- 8.5 - 9.5
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- nominal concentration:
4.6, 10, 22, 46, 100 mg/L
calculated concentration (based on measured nitrogen and ammonia concentration):
4.5, 9.7, 21.7, 46, 98.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Type: closed
- Material, fill volume: glass, 65 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water as demanded in the guidelines
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours, using neon tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation every 24 hours
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes. The 24h-EC50 value was determined as 1.8 mg/L, lying within the range of 0.6 - 2.1 mg/L which is mentioned in the OECD guideline.
- Reported statistics and error estimates:
- The estimation of the biological results was accomplished using the software ToxRat® Professional, version 3.2.1.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 48 h immobilisation study with Daphnia magna, an EC50 of 22 mg/L and a NOEC value of 10 mg/L was determined.
- Executive summary:
Short term toxicity of the test substance was determined according to OECD guideline 202. One valid experiment was performed. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 daphnia were exposed (4 replicates á 5 animals) to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
The three highest concentrations showed toxicity between 55 and 100 % immobilisation. None of the animals were immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable. At the start and at the end of the test, the content of the test item in the test solutions was determined by NH4+-determination using a photometric method. The measured concentrations laid between 97 % and 100 % of the nominal concentrations at the beginning of the test and between 96 % and 100 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentrations.
The following results were determined for the test item lithium amide (species: Daphnia magna).
48h-NOEC = 10 mg/L
48h-LOEC = 22 mg/L
48h-EC50 = 22 mg/L
Reference
The three highest concentrations showed toxicity between 55 and 100% immobilisation. None of the animals were immobilised in the blank control.
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours | ||||||||
absolute | in % | absolute | in % | |||||||
Blank control |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
4.6 |
0 |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
22 | 0 | 1 | 2 | 1 | 20 | 3 | 3 | 3 | 2 | 55 |
46 | 5 | 5 | 5 | 5 | 100 | 5 | 5 | 5 | 5 | 100 |
100 | 5 | 5 | 5 | 5 | 100 | 5 | 5 | 5 | 5 | 100 |
Description of key information
In a 48 h immobilisation study with Daphnia magna, an EC50 of 22 mg/L and a NOEC value of 10 mg/L was determined with the source substance lithium amide.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 22 mg/L
Additional information
Short term toxicity of the test substance was determined according to OECD guideline 202. One valid experiment was performed. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 daphnia were exposed (4 replicates á 5 animals) to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
The three highest concentrations showed toxicity between 55 and 100 % immobilisation. None of the animals were immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable. At the start and at the end of the test, the content of the test item in the test solutions was determined by NH4+-determination using a photometric method. The measured concentrations laid between 97 % and 100 % of the nominal concentrations at the beginning of the test and between 96 % and 100 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentrations.
The following results were determined for the test item lithium amide (species: Daphnia magna).
48h-NOEC = 10 mg/L
48h-LOEC = 22 mg/L
48h-EC50 = 22 mg/L
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