Registration Dossier
Registration Dossier
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EC number: 236-031-3 | CAS number: 13106-76-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from Secondary literature
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Gene mutation in vitro toxicity assay for Ammonium molybdate- Molybdenum and molybdenum compounds
- Author:
- Gene mutation in vitro toxicity assay for Ammonium molybdate- Molybdenum and molybdenum compounds
- Year:
- 2 013
- Bibliographic source:
- Health Council of Netherlands, U-7988/JR/fs/459-B69, 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- In vitro mammalian chromosome aberration test was performed to evaluate the mutagenic nature of ammonium molybdate
- GLP compliance:
- not specified
- Type of assay:
- other: Chromosomal aberration assay
Test material
- Reference substance name:
- Ammonium molybdate(VI)
- EC Number:
- 236-031-3
- EC Name:
- Ammonium molybdate(VI)
- Cas Number:
- 13106-76-8
- Molecular formula:
- H4N.1/2MoO4
- IUPAC Name:
- ammonium molybdate(VI)
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: Ammonium molybdate
- Molecular formula: MoO42H4N
- Molecular weight: 196.0132 g/mol
- Substance type: Inorganic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data
Method
- Target gene:
- No data
Species / strain
- Species / strain / cell type:
- lymphocytes: Human
- Details on mammalian cell type (if applicable):
- No data
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- not specified
- Metabolic activation system:
- No data
- Test concentrations with justification for top dose:
- 10 µM
- Vehicle / solvent:
- No data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: No data
DURATION
- Preincubation period: No data
- Exposure duration: 24 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data
SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data
NUMBER OF REPLICATIONS: No data
NUMBER OF CELLS EVALUATED: No data
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data
OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data
OTHER: No data - Rationale for test conditions:
- No data
- Evaluation criteria:
- The slides were observed for induction of chromosome aberrations
- Statistics:
- No data
Results and discussion
Test results
- Species / strain:
- lymphocytes: Human
- Metabolic activation:
- not specified
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No data
Applicant's summary and conclusion
- Conclusions:
- Ammonium molybdate induced chromosome aberrations in human lymphocytes and hence is likely to be mutagenic in vitro.
- Executive summary:
In vitro mammalian chromosome aberration test was performed to evaluate the mutagenic nature of ammonium molybdate. The study was performed at dose level of 10µM using human lymphocytes. The duration of exposure was 24 hrs. Ammonium molybdate induced chromosome aberrations in human lymphocytes and hence is likely to be mutagenic in vitro.
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