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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study undertaken and reported scientifically but not to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 433 draft (Acute Inhalation Toxicity: Fixed Concentration Procedure) (not officially approved)
Deviations:
not specified
GLP compliance:
no
Test type:
fixed concentration procedure

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride
EC Number:
204-077-3
EC Name:
1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride
Cas Number:
115-27-5
Molecular formula:
C9H2Cl6O3
IUPAC Name:
3-({3-carboxy-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carbonyloxy}carbonyl)-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carboxylic acid
Details on test material:
- Name of test material (as cited in study report): Chlorendic anhydride- Physical state: Fine white powder containing some chunks.- Analytical purity: 93.8%- Lot/batch No.: 3-12-206

Test animals

Species:
rat
Strain:
other: CD
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS- Source: Charles River- Age at study initiation: - Weight at study initiation: males, 211 to 226 g; female, 213 to 232 g- Fasting period before study: No- Housing: Wire-mesh cages.- Diet: Purina laboratory chow, ad libitum - Water: ad libitum- Acclimation period: 1 weekENVIRONMENTAL CONDITIONS- Temperature (°C): - Humidity (%): - Air changes (per hr): - Photoperiod (hrs dark / hrs light): IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:
inhalation: dust
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION- Exposure apparatus: Glass chamber- Exposure chamber volume: 160 litre- Method of holding animals in test chamber: Wire-mesh exposure cages.- Source and rate of air: rotary centrifugal air-pump located at the exhaust side of the chamber at 7.5 litres/min.- Method of conditioning air: - System of generating particulates/aerosols:Dispersing powder at a calculated rate.- Method of particle size determination: - Treatment of exhaust air:- Temperature, humidity, pressure in air chamber: TEST ATMOSPHERE- Brief description of analytical method used: - Samples taken from breathing zone: yes/noVEHICLE- Composition of vehicle (if applicable):- Concentration of test material in vehicle (if applicable):- Justification of choice of vehicle: - Lot/batch no. (if required): - Purity: TEST ATMOSPHERE (if not tabulated)- Particle size distribution:- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): CLASS METHOD (if applicable)- Rationale for the selection of the starting concentration:
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
1 h
Concentrations:
A total of 91.37 grams of the powders was disseminated during the 1 hour exposure period. The rate of powder dissemination was calculated to be 1.52 grams per minute. The rate of chamber airflow was 7.5 liters per minute and the chamber dust concentration was calculated to be 203.0 mg/l. The chamber atmosphere was extremely dusty.
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days - Frequency of observations and weighing: Twice daily - Necropsy of survivors performed: no- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 203 mg/L air
Based on:
other: Mortality
Exp. duration:
1 h
Mortality:
None
Clinical signs:
other: The immediate response of the rats to the experimental atmosphere was an increase of activity in preening. After several minutes of exposure this activity decreased. After 30 minutes of exposure six rats exhibited salivation. By the end of the exposure
Body weight:
A slight body weight loss was observed in 6 rats for 1 day post-exposure.
Gross pathology:
None

Applicant's summary and conclusion

Interpretation of results:
relatively harmless
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
Inhalation exposure of 10 rats for 1 hour to a dust atmosphere of Chlorendic Anhydride at a metered concentration of 203.0 mg / l resulted in salivation during the exposure. A slight body weight loss was observed in 6 rats for 1 day post-exposure.