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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study undertaken and reported scientifically but not to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.1 bis (Acute Oral Toxicity - Fixed Dose Procedure)
Deviations:
not specified
GLP compliance:
no
Test type:
fixed dose procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride
EC Number:
204-077-3
EC Name:
1,4,5,6,7,7-hexachloro-8,9,10-trinorborn-5-ene-2,3-dicarboxylic anhydride
Cas Number:
115-27-5
Molecular formula:
C9H2Cl6O3
IUPAC Name:
3-({3-carboxy-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carbonyloxy}carbonyl)-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carboxylic acid
Details on test material:
- Name of test material (as cited in study report): Chlorendic anhydride- Analytical purity: 98.81%- Lot/batch No.: 3-12-206

Test animals

Species:
rat
Strain:
other: Charles River CD
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS- Source: Charles River Breeding Laboratories, Inc., Portage, Michigan- Age at study initiation: - Weight at study initiation: 200 to 232 g- Fasting period before study: Overnight period immediately preceding oral administration during which food but not water was withheld- Housing: Hanging wire mesh cages.- Diet: Purina laboratory chow ad libitum- Water: ad libitum- Acclimation period:ENVIRONMENTAL CONDITIONS- Temperature (°C): - Humidity (%): - Air changes (per hr): - Photoperiod (hrs dark / hrs light): IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Details on oral exposure:
VEHICLE- Concentration in vehicle: 807.1, 1281, 2034, 3229 and 5126 mg/kg- Amount of vehicle (if gavage): 10 ml/kg- Justification for choice of vehicle:- Lot/batch no. (if required): - Purity: MAXIMUM DOSE VOLUME APPLIED: DOSAGE PREPARATION (if unusual):CLASS METHOD (if applicable)- Rationale for the selection of the starting dose:
Doses:
807.1, 1281, 2034, 3229 and 5126 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days - Frequency of observations and weighing: Immediately before dosing, 7 days and 14 days.- Necropsy of survivors performed: yes- Other examinations performed: clinical signs, body weight,histopathology

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
2 562 mg/kg bw
95% CL:
2 218 - 2 961
Sex:
female
Dose descriptor:
LD50
Effect level:
2 130 mg/kg bw
95% CL:
1 698 - 2 671
Sex:
male/female
Dose descriptor:
LD50
Effect level:
2 336 mg/kg bw
95% CL:
2 065 - 2 643
Mortality:
2034 mg/kg - 2 female3229 mg/kg - 5 male; 5 female5126 mg/kg - 5 male; 5 female
Clinical signs:
At all dose rates, diarrhoea was evident. At 2034 mg/kg and above, hypoactivity, ataxia and alopecia were noted.
Body weight:
All animals which survived the dosing increased their body weight.

Applicant's summary and conclusion

Interpretation of results:
relatively harmless
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
As the LD50 > 2000 mg/kg for both sexes this substance is not classified as harmful.