Sulphuric diamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sulphuric diamide

EC Number:

232-262-9

EC Name:

Sulphuric diamide

Cas Number:

7803-58-9

Molecular formula:

H4N2O2S

IUPAC Name:

Sulphuric diamide

Test material form:

solid: crystalline

Specific details on test material used for the study:

Manufacturer: Anhui Shindo Chemical Co. LTD.Batch number: 0106A26 (A01151230)Physical state: Solid, crystalline substanceColour: WhiteStorage: In a dry place, protected from light

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

The preparation of stock and test solutions was based on the preliminary non GLP range finding test. For the stock solution 200.08 mg of test item was measured into 1 000 ml Elendt M4 medium. Six dilution levels were prepared by mixing appropriate volume of Elendt M4 medium and stock solution of the test item.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test species: Daphnia magna StrausAge: < 24 hours neonatesOrigin: National Institute of Environmental HealthCulture conditions: The test species is maintained in Elendt M4 medium with culture conditions at 21±1 °C and a light/dark cycle of 16/8 hours with light intensities of less than 1000 Lux. The cultures are fed with centrifugalized green alga (Pseudokirchneriella subcapitata) cells daily at weekdays.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

The number of immobilised and abnormally behaved daphnids was recorded after 24 and 48h.

Test conditions

Test temperature:

The temperature of thermostat was recorded daily and registered continuously by Extech datalogger.Temperature at the start of the test: 21.1 °CTemperature after 24 hours: 21.1 °CTemperature at the end of the test: 21.3 °CThe temperature inside the thermostat at the test vessels was measured and registered continuously by Extech SD200 thermometer and datalogger.

pH:

The pH was measured at the beginning and at the end of the test. At the beginning of the test the pH was measured from the remaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the pH was measured in each test vessel.There was not any significant change in the pH during the test.

Dissolved oxygen:

The dissolved oxygen was measured at the beginning and at the end of the test. At the beginning of the test the dissolved oxygen was measured from the remaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the dissolved oxygen was measured in each test vessel. There was not any significant change in oxygen concentration during the test.

Nominal and measured concentrations:

In the test the following nominal concentrations of test solutions were applied: 200; 160; 120; 80; 40 and 20 mg/L.

Details on test conditions:

The test was maintained under static conditions for a period of 48 hours. At the start of the study each test vessels contained 50 ml of test solutions and five young daphnids. In the test four replicates total of 20 animals at each test concentration level were investigated. The test vessels were covered with plastic lids and were kept in thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle.Each control vessels containing five young daphnids in Elendt M4 medium were prepared in four replicates and kept under the same static conditions.The number of immobilised and abnormally behaved daphnids was recorded after 24 and 48h. A daphnia is considered to be immobile if it is not able to swim within 15 seconds after a gentle agitation of the test vessel.The daphnids were not fed during the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 200 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 200 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Acute toxicity expressed as the effective concentrations ECx which immobilizes the x% of the daphnids were calculated by the ToxRat® statistic software. As immobilization of the test item to Daphnia did not exceed 5% the ECx values were not determined.

Applicant's summary and conclusion

Conclusions:

In this Daphnia sp., Acute Immobilisation Test for Sulfamide the results indicated that the test item is not hazardous to the aquatic environment [48 hr EC50 (for Crustacea) > 100 mg/L].