1,4:5,8-Dimethanonaphthalene, 1,2,3,4,4a,5,8,8a-octahydro-, (1R,4S,4aR,5S,8R,8aS)-rel-

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 September 2011 - 24 June 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to OECD test guidelines and in compliance with GLP

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Test temperature:

25°C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Liquid chromatograph
-Column: HyperClone CN (CPS) (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm

MOBILE PHASES
- Type:Methanol:water (55:45 v/v)
- pH: not applicable the test substance is non-ionisable
- Solvents for dissolving test and reference substances: HPLC mobile phase

DETERMINATION OF DEAD TIME
- Method: internal standard (formamide) with no retention on the HPLC column

REFERENCE SUBSTANCES
- Identity: Acetanilide; Nitrobenzene; Naphthalene; 2-Methylnaphthalene Anthracene; DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: approximately 1 mg/l
- Quantity of reference substances: 0.09 - 0.4 mg/ml


REPETITIONS
- Number of determinations: duplicate

EVALUATION
- Calculation of capacity factors k': measured retention time - dead time/deadtime
- Calculation of retention times: measured retention time
- Determination of the log Koc value: A calibration plot of log10k versus log10Koc was constructed for the reference standards. The logarithm of the capacity factor for the test substance was then calculated and the log10Koc value was determined by interpolation.

Key result

Type:

log Koc

Value:

>= 4 - <= 4 dimensionless

Temp.:

25 °C

Details on results (HPLC method):

- Retention times of reference substances used for calibration (minutes):
Acetanilide: 3.53
Nitrobenzene: 4.05
Naphthalene: 4.88
2-Methylnaphthalene: 5.41
Anthracene: 7.18
DDT: 11.7

- Details of fitted regression line (log k' vs. log Koc): Linear regression (based on mean of duplicate standard runs): log10Koc = 3.47 log10k + 3.99
- Average bretention time for deadtime sdtandard: 3.061
- Average retention data for test substance: 6.175

Validity criteria fulfilled:

yes

Conclusions:

TCD was found to have an adsorption coefficient (log10Koc) of 4.0.

Description of key information

TCD was found to have a soil adsorption coefficient  log10Koc of 4.0 (Koc 10,000)  at 25°C.

Key value for chemical safety assessment

Koc at 20 °C:

4

Additional information

A soil adsorption coefficient determination was conducted as part of a package of physico-chemical testing for TCD (Huntingdon Life Sciences, 2013, Study number VHJ0054). The testing was conducted in compliance with GLP, and the adsorption coefficient determination was performed according to OECD test guideline 121 and EC test method C.19.

TCD was found to have a soil adsorption coefficient log10Koc of 4.0 (Koc 10,000) at 25°C.