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Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of assay:
bacterial reverse mutation assay

Test material

Specific details on test material used for the study:
Purity of test material > 99 %

Method

Target gene:
In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Metabolic activation system:
rat liver homogenate fraction( " S-9")
Test concentrations with justification for top dose:
The dose range of 6 different doses from 4 µg/plate to 10 000 µg/plate was used.
Vehicle / solvent:
Acetone
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
Positive controls:
yes
Positive control substance:
9-aminoacridine
2-nitrofluorene
sodium azide
other: N-Methyl-N'-nitro-N-nitrosoguanidine
Details on test system and experimental conditions:
Mutagenicity Test: Top agar is prepared for Salmonella strain. Bacteria are grown overnight in nutrient broth.The suitable amount of bacteria in cell suspension is checked by nephelmetry. For inoculation, stock cultures which are stored at -80 °C are used.

Results and discussion

Applicant's summary and conclusion

Conclusions:
2,4-Dichlorbenzoyl chloride is not mutagenic in these bacterial test system either with or without exogenous metabolic activiation at the dose level investigated.