Registration Dossier
Registration Dossier
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EC number: 203-400-5 | CAS number: 106-46-7
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline not defined. No GLP study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: other: Bone marrow micronucleus test
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,4-dichlorobenzene
- EC Number:
- 203-400-5
- EC Name:
- 1,4-dichlorobenzene
- Cas Number:
- 106-46-7
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,4-dichlorobenzene
- Details on test material:
- IUCLID4 Test substance: as prescribed by 1.1 - 1.4
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: unspecified
- Duration of treatment / exposure:
- 2 administrations at an interval of 24 h.
- Frequency of treatment:
- no data
- Post exposure period:
- no data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
2 x 177.5 or 355 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 10 animals-negative control.
10 animals - treatment with 2x 177.5 mg/kg.
10 animals - treatment with 2x 355 mg/kg. - Control animals:
- yes
Examinations
- Tissues and cell types examined:
- Polychromatic erythrocytes from bone marrow.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
Any other information on results incl. tables
RS-Freetext:
negative
Applicant's summary and conclusion
- Executive summary:
Employing the micronucleus test, p-Dichlorobenzene was investigated in male and female mice for a possible clastogenic effect on the chromosomes of bone marrow erythroblasts.
The treated mice recieved two intraperitoneal administrations at an interval of 24 hours.
6 hours after the second administration the femoral marrow was prepared. The doses of p-Dichlorobenzene were 2x 177.5 mg/kg and 2x355 mg/kg.
The animals treated with p-Dichlorobenzene showed no symptoms of toxicity after the administration.
No deaths related to the substance occured.
The ratio of polychromatic to normochromatic erythrocytes was slightly altered in the 2x355 mg/kg group.
No indications of a clastogenic effect of p-Dichlorobenzene were found after treatment with 2x 177.5 mg/kg and 355 mg/kg per os.
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