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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Rating in accordance with OECD SIDS (2002)

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2001
Reference Type:
secondary source
Title:
SIDS - C.I. PIGMENT YELLOW 53
Author:
OECD
Year:
2002
Bibliographic source:
UNEP PUBLICATIONS

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Antimony nickel titanium rutile
EC Number:
701-459-6
Cas Number:
8007-18-9
Molecular formula:
Ni(x/3)Sb(2x/3)Ti(1-x)O2 0,1≤x≤0,3
IUPAC Name:
Antimony nickel titanium rutile
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: C.I. Pigment Yellow 53 was almost insoluble in water and showed a low toxicity by preliminary test. Consequently, the experiments were carried out with 1.00 mg/L at which precipitates and floating substances were not observed. However, it was likely that the test substance was not dissolved, because the presence of the test substance in the filtrate (the test solution was filtered with 0.45 µm membrane filter) was below the detection limit of ICP-MS operating condition.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS
- Source: National Institute for Environmental Studies (JAPAN)
- Age at study initiation: < 24 h

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20.4 - 20.5 ºC
pH:
8.2 - 8.4 (control)
Dissolved oxygen:
8.5 - 8.7 mg/L
Nominal and measured concentrations:
Nominal Concentrations: control and 1.00 mg/L
1000 mg/L stock solution was prepared for 1.00 mg/L tests as followed: 100 mg test substance was added in 100 mL dilution water. The test substance was not dissolved but dispersed in stock and test solutions.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL test solution in a 100 mL-Glass Beaker with Teflon-lined sheet cap
- Renewal rate of test solution: Every 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 (OECD Guideline 211)

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, light-darkness cycle
- Light intensity: <= 800 lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
dispersed
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
dispersed
Basis for effect:
mobility
Reported statistics and error estimates:
- Statistical Method: Binomial
a) Data Analysis: The EC50 value and its 95% confidence limits were not determined, a limit test (OECD TG 202) was conducted.
b) Method of Calculating Mean Measured Concentrations (i.e.arithmetic mean, geometric mean, etc.): Geometric Mean

Any other information on results incl. tables

- Measured Concentrations:


The tested concentrations were measured at 0 hour and 24 hours later (before exchange of test solution).


The tested concentration at 24 hours was shown as 48% of nominal concentration; however it was likely that the measured concentration did not reflect really C. I. Pigment Yellow 53 content in the tested solution due to the low solubility.


------------------------------------------------------------------------------------------------


Nominal                        Measured                 Mean* measured


concentration               concentration           concentration


mg/L                              mg/L                          mg/L


------------------------------------------------------------------------------------------------           


                         0 Hour (new)  24 Hours (old)


 -----------------------------------------------------------------------------------------------


Control               <0.05           <0.05                     –


1.0                        0.92              0.48                     0.63


------------------------------------------------------------------------------------------------


 *: Geometric Mean


 


 - Calculation of toxic values:


The calculation of the toxic values is based on the nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Antimony nickel titanium rutile is not acutely toxic to Daphnia magna in a test according to OECD 202 with suspensions prepared with nominal concentrations far exceeding its water solubility and the respective EC50 for immobilisation is estimated with > 1 mg/L (nominal, dispersed).