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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The 30-min EC10 value for monoethanolamine in domestic activated sludge is >1,000 mg/L, the 16-h toxicity threshold (equivalent to an EC3 value) for citric acid is >10,000 mg/L. After correction for molecular weight the effect concentrations for tris[(2-hydroxyethyl)ammonium] citrate are >6,146 and >19,537 mg/L, respectively. The lowest value of >6,146 mg/L will be used in the assessment.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
6 146 mg/L

Additional information

No respiration inhibition tests with activated sludge are available for tris[(2-hydroxyethyl)ammonium] citrate. Tris[(2-hydroxyethyl)ammonium] citrate is the acidic salt of monoethanolamine and citric acid. Monoethanolamine and citric acid are the starting materials for the synthesis of tris[(2-hydroxyethyl)ammonium] citrate, and the only difference is that in tris[(2-hydroxyethyl)ammonium] citrate the substances are present in their ionic forms as a monoethanolamine cation and a citrate anion. It is expected that the ecotoxicological properties of tris[(2-hydroxyethyl)ammonium] citrate shall be governed by the properties of monoethanolamine and citric acid (for more details see reporting format for the analogue approach in Appendix A.2).

A non-GLP OECD guideline 209 study with domestic activated sludge is available for monoethanolamine (BASF, 1991). The 30-min EC10 value is >1,000 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is >6,146 mg/L.

For citric acid a non-GLP growth inhibition study (Bringmann and Kühn, 1977) with Pseudomonas putida. The 16-h toxicity threshold (equivalent to an EC3 value) is >10,000 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is >19,537 mg/L.

The effect concentration of >6,146 mg/L will be used in the assessment.