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Ecotoxicological information

Additional ecotoxological information

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Administrative data

Endpoint:
additional ecotoxicological information
Remarks:
Specific investigations: Biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
supporting study

Data source

Reference
Reference Type:
publication
Title:
In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryos
Author:
Egloff C, Crump D, Chiu S, Manning G, McLaren KK, Cassone CG, Letcher RJ, Gauthier LT, Kennedy SW
Year:
2011
Bibliographic source:
Toxicol Lett., 207, 25-33 (2011)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Several brominated flame retardants were administrated to chicken embryonic hepatocytes (CEH) to determine cytotoxicity and changes in relative mRNA abundance of 11 genes involved in xenobiotic metabolism, lipid metabolism and thyroid hormone (TH) homeostasis by real-time reverse transcription-PCR.
GLP compliance:
no
Type of study / information:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2-ethylhexyl) tetrabromophthalate
EC Number:
247-426-5
EC Name:
Bis(2-ethylhexyl) tetrabromophthalate
Cas Number:
26040-51-7
Molecular formula:
C24H34Br4O4
IUPAC Name:
1,2-bis(2-ethylhexyl) 3,4,5,6-tetrabromobenzene-1,2-dicarboxylate
Specific details on test material used for the study:
Purity > 98 %

Results and discussion

Any other information on results incl. tables

Test concentrations in the range of 7.06 and 21.2 mg/L of bis(2-ethylhexyl) tetrabromophthalate (BEHTBP) did not affect hepatocyte viability.

Applicant's summary and conclusion

Conclusions:
Test concentrations in the range of 7.06 and 21.2 mg/L of bis(2-ethylhexyl) tetrabromophthalate (BEHTBP) did not affect hepatocyte viability.
Executive summary:

In this study in vitro effects of bis(2-ethylhexyl) tetrabromophthalate (BEHTBP) with respect to hepatic mRNA expression in chicken embryos (Gallus gallus domesticus) was investigated. In the the study primary cultures of chicken embryonic hepatocytes (CEH) were used. It was reported that test concentrations in the range of 7.06 and 21.2 mg/L of bis(2-ethylhexyl) tetrabromophthalate  (BEHTBP) did not affect hepatocyte viability.