Registration Dossier
Registration Dossier
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EC number: 281-719-9 | CAS number: 84029-45-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed study according to acknowledged guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 part 25 (1984)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate
- EC Number:
- 281-719-9
- EC Name:
- Sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate
- Cas Number:
- 84029-45-8
- Molecular formula:
- C14H12N2O6S.Na
- IUPAC Name:
- sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate
- Details on test material:
- purity: 87 - 90 %
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial, adapted
- Details on inoculum:
- Mixture of activated sludge from the sewage treatment plant of the Hoechst AG at Frankfurt am Main, Höchst with activated sludge from the sewage treatment plant at Hoechst AG, site Kelsterbach in a ratio of 1 to 2.
Washing the sediment from activated sewage sludge two times by centrifuging and resuspending in mineral medium - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 465 mg/L
- Based on:
- test mat.
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 12
- Sampling time:
- 1 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 26
- Sampling time:
- 6 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 21
- Sampling time:
- 13 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 13
- Sampling time:
- 20 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 9
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Diethylene glycole: 95 % biodegradation after 27 days (DOC)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Nitrotolylsulfanilic-acid, Na-salt was not inherently biodegradable under the conditions of this test.
- Executive summary:
Testing of Nitrotolylsulfanilic-acid, Na-salt for inherent biodegradability was performed in the ZahnWellens test. After 28 days only a degradation of about 9% was oberserved.
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