Registration Dossier
Registration Dossier
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EC number: 207-434-1 | CAS number: 471-01-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study well documented, meets generally accepted scientific principles, acceptable for assessment. Data were taken from the structural analogue beta-Isophoron, CAS 78-59-1. The structural analogue differs in the location of the carbon double bound within the ring structure of the molecule, whereas the rest of the molecule is identical to CAS 471-01-2.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the test method -Activated sludge, respiration inhibition test proposed by the OECD
- Author:
- Yoshioka Y, Nagase H, Ose Y, Sato T
- Year:
- 1 986
- Bibliographic source:
- Ecotoxicol. Environ. Safety 12, 206-212
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-en-1-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- TEST ORGANISMS
- Origin: Gifu-Hokubu Sewage Treatment Plant
- Pretreatment: washing with dist. water >= 3 times, suspended in dist. water at 4 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
Test conditions
- Test temperature:
- 20 °C
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
