Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
- Source and lot/batch No.of test material: L2018-005
- Expiration date of the lot/batch: 01 Apr 2020
- Purity: 99.8 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 48 hours

Test solutions

Vehicle:
no
Details on test solutions:
The definitive test was conducted at the nominal test substance concentration of 100 mg/L. A control treatment was also included in the test.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus, Clone V, was used as the test organism. The animals are continuously
bred in the laboratory and were originally purchased in a healthy condition from the Federal
Environment Agency in Berlin/Germany. Freshly hatched daphnids less than 24 hours old and not first brood progeny were used for the
test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19.1-19.9°C
pH:
7.04 - 7.85
Dissolved oxygen:
>=6.4 mg/l
Nominal and measured concentrations:
The measured content of test item was 95 % of nominal in the fresh sample. In the aged sample
the measured content was 105 % of nominal. Therefore toxicological endpoints were evaluated
using the nominal concentrations of the test item.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes