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EC number: 208-051-2 | CAS number: 506-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyanogen bromide
- EC Number:
- 208-051-2
- EC Name:
- Cyanogen bromide
- Cas Number:
- 506-68-3
- Molecular formula:
- CBrN
- IUPAC Name:
- cyanogen bromide
- Test material form:
- solid: particulate/powder
Constituent 1
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain:
- Source (laboratory, culture collection): Culture maintained in BRF, India
- Age of inoculum (at test initiation):
- Method of cultivation:
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- pH:
- Range Finding
0 h : Control: 8.10
Treatment: 7.63 – 7.87
72 h : Control: 8.10
Treatment: 7.47 – 7.83
Main Study
0 h : Control: 8.10
Treatment: 7.70 – 7.97
72 h : Control: 8.10
Treatment: 7.63 – 7.97 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Conical flask
- Material, size, headspace, fill volume: 250 mL
- Initial cells density: >5×104 cells/ml
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3 replications/ Concentrations
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
GROWTH MEDIUM
- Standard medium used: yes/no
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod:
- Light intensity and quality:
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.708 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- other: probit analysis method
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the growth rate inhibition (%) result, the EC50 value of Cyanogen Bromide for 72 hour period was 0.708 mg/l (Lower Limit 0.512 – Upper Limit 0.904) by using Probit analysis method.
- Executive summary:
Growth inhibition effect of Cyanogen Bromide Sponsored by Libor Bachura vyzkum a vyroba was tested againstPseudokirchneriella subcapitataat Bioscience Research Foundation, India by following OECD guideline No. 201, Adopted 23 March 2006.
Solubility and Stability Analysis
The test substance 100 mg wasdissolved in 1000 ml of exposure medium and found that it was soluble. Hence exposure medium was used as control. The test substance was stable in the exposure medium for 72 hours. Hence the study was conducted as static test.
Range Finding Study
Prior to exposure for the range finding study the preculture was maintained for 3 days in test condition. The initial cell concentration of 5x103- 5x104cells/ml was inoculated to control and treatment of the OECD 201 medium. The cells were counted at 24, 48 and 72 h after exposure period by using haemocytometer under the microscope.During the study,the pH of the medium was between pH 7.47 - 8.10.
A range finding study was conducted with control, 0.1, 1, 10, 50 and 100 mg/l concentration. On the day of exposure the initial average pH of the medium was 8.10, 7.87, 7.83, 7.73, 7.67 and 7.63 recorded in control, 0.1, 1, 10, 50 and 100 mg/l concentration respectively.
At the end of 24 hour observation, the average specific growth rate of the control and treatment was 5.95, 4.58, 4.18, 1.87, 1.99 and 1.23recorded in control,0.1, 1, 10, 50 and 100 mg/lconcentrationrespectively. At the end of 48 hour observation, the average specific growth rate was 6.36, 5.12, 5.29, 2.15, 2.08 and 1.54 recorded in control, 0.1, 1, 10, 50 and 100 mg/lconcentrationrespectively. At the end of 72 hour observation, the average specific growth rate was 7.27, 6.75, 5.02, 1.78, 0.00 and 0.00 recorded in control, 0.1, 1, 10, 50 and 100 mg/l concentration respectively. At the end of experimental period 0-3 day, the average specific growth rate was 2.42, 2.25, 1.67, 0.59, 0.00 and 0.00 in control, 0.1, 1, 10, 50 and 100 mg/l concentration per day.
At the end of 24 hour observation, the percentage of growth inhibition was 23.09%, 29.80%, 68.65%, 66.56% and 79.34% observed in 0.1, 1, 10, 50 and 100 mg/l concentration respectively. At the end of 48 hour observation, 19.53%, 16.87%, 66.22%, 67.23% and 75.87% of growth inhibition was observed in exposed concentrations of 0.1, 1, 10, 50 and 100 mg/l concentration respectively. At the end of 72 hour observation, 7.14%, 31.00%, 75.48%, 100.00% and 100.00% of growth inhibition was observed in exposed concentration of 0.1, 1, 10, 50 and 100 mg/l concentration respectively. The percentage of growth inhibition on 0-3rdday was 7.14%, 31.00%, 75.48%, 100.00% and 100.00% observed in exposed concentrations of 0.1, 1, 10, 50 and 100 mg/l concentration respectively.
At the end of 0 – 3 day 40.50%, 89.53%, 99.65%, 100.00% and 100.00% yield reduction was observed in 0.1, 1, 10, 50 and 100 mg/l exposed concentration respectively. The final day average pH of the medium was 8.10, 7.83, 7.73, 7.67, 7.60 and 7.47 in control, 0.1, 1, 10, 50 and 100 mg/l exposed concentration respectively.
Main Study
Based on the results of range finding study, main study was conducted with control and five different concentrations of0.0625, 0.125, 0.25, 0.5 and 1mg/l concentrations. On the day of exposure the initial average pH of medium was 8.10, 7.97, 7.90, 7.77, 7.73 and 7.70 recorded in control, 0.0625, 0.125, 0.25, 0.5 and 1 mg/l exposed concentration respectively.
At the end of 24 hour observation, average specific growth rate was 6.07, 5.30, 4.81, 4.59, 4.18 and 4.04recorded in control, 0.0625, 0.125, 0.25, 0.5 and 1 mg/l concentrationrespectively. At the end of 48 hour observation, the average specific growth rate was 6.29, 5.27, 4.65, 4.56, 4.45 and 3.29 observed incontrol, 0.0625, 0.125, 0.25, 0.5 and 1 mg/l exposed concentrationrespectively. At the end of 72 hour observation, the average specific growth rate was 6.70, 4.90, 4.78, 4.20, 4.18 and 2.62 recorded incontrol, 0.0625, 0.125, 0.25, 0.5 and 1 mg/lconcentration of exposed test substance respectively. At the end of experimental period, 0-3 day, the average specific growth rate was 2.23, 1.63, 1.59, 1.40, 1.39 and 0.87 incontrol, 0.0625, 0.125, 0.25, 0.5 and 1 mg/lconcentration respectively.
At the end of 24 hour observation, the percentage of growth inhibition was 12.78, 20.85, 24.49, 31.09 and 33.44% observed in0.0625, 0.125, 0.25, 0.5 and 1mg/l concentration respectively. At the end of 48 hour observation, 16.21, 26.14, 27.54, 29.25 and 47.75% of growth inhibition was observed in exposed concentrations of0.0625, 0.125, 0.25, 0.5 and 1mg/l concentration respectively. At the end of 72 hour observation, 26.89, 28.66, 37.32, 37.67 and 53.01% of growth inhibition was observed in exposed concentration of0.0625, 0.125, 0.25, 0.5 and 1mg/l concentration respectively. The percentage of growth inhibition on 0-3rdday was 26.89, 28.66, 37.32, 37.67 and 60.93% observed in exposed concentrations of0.0625, 0.125, 0.25, 0.5 and 1mg/l concentration respectively.
At the end of 0 – 3 day, 83.50%, 85.34%, 91.91%, 92.08% and 98.40% yield reduction was observed in0.0625, 0.125, 0.25, 0.5 and 1mg/l in exposed concentration of the test substance respectively. The final day average pH of the medium was 8.10, 7.97, 7.80, 7.73, 7.67 and 7.63 recorded in control,0.0625, 0.125, 0.25, 0.5 and 1mg/l exposed concentration respectively.
Coefficient variation (%)
The coefficient variation of the control culture were 0.00, 0.59, 0.95 and 0.39 for 0-1, 1-2, 2-3 and 0-3 day respectively section by section within the stipulated limit as per OECD 201.
Effective concentration for growth inhibition %
Based on the result of growth rate inhibition (%), the EC50value was 0.708 mg/l (Lower Limit 0.512 – Upper Limit 0.904) forCyanogen Bromideon alga.
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