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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Secondary effluent from Lulu Island Domestic Wastewater Treatment plant, Richmond, BC
Duration of test (contact time):
28 d
Initial conc.:
10 mg/L
Based on:
test mat.
Reference substance:
benzoic acid, sodium salt
Preliminary study:
Test material did not pass out of the acclimation phase during the evaluation.
Key result
Parameter:
% degradation (O2 consumption)
Value:
0.6
Sampling time:
7 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
0.8
Sampling time:
14 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
-0.6
Sampling time:
21 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
-0.2
Sampling time:
24 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
-1.6
Sampling time:
28 d
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Under the conditions of this study, no significant biodegradation was observed.
Executive summary:

The potential for the test material to undergo biodegradation was investigated in a closed bottle test conducted in accordance with OECD 301 under GLP conditions.

Non-adapted activated sludge was exposed to the test material under aerobic conditions for 28 days.

Under the conditions of this study, no significant biodegradation was observed.

Description of key information

Under the conditions of the study the test material was determined to be not readily biodegradable.

In accordance with section 9.2 of Column 2 of REACH Annex IX, further degradation studies are only needed if indicated as necessary based on the result of Chemical Safety Assessment.  For this registration, the Chemical Safety Assessment does not indicate a need for such further studies. Therefore, it is considered justified to omit testing for the identification of degradation products.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The potential for the test material to undergo biodegradation was investigated in a closed bottle test conducted in accordance with OECD 301 under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

Non-adapted activated sludge was exposed to the test material under aerobic conditions for 28 days.

Under the conditions of this study, no significant biodegradation was observed.