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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: inhalation

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Administrative data

Endpoint:
short-term repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Referred in a recognized source of peer reviewed scientific data on chemicals.

Data source

Reference
Reference Type:
review article or handbook
Title:
Subchronic inhalation toxicity of ethylbenzene in mice, rats, and rabbits.
Author:
Cragg, S.T., Clarke, E.A., Daly, I.W., Miller, R.R., Terrill, J.B., and Quellette, R.E.
Year:
1989
Bibliographic source:
Fundam. Appl. Toxicol. 13, 399-408.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.8 (Subacute Inhalation Toxicity: 28-Day Study)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylbenzene
EC Number:
202-849-4
EC Name:
Ethylbenzene
Cas Number:
100-41-4
Molecular formula:
C8H10
IUPAC Name:
ethylbenzene

Test animals

Species:
other: Mice, Rats, and Rabbits

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Duration of treatment / exposure:
4 weeks
Frequency of treatment:
6 hr/day, 5 days/week
No. of animals per sex per dose:
5

Results and discussion

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Study observed:

 No chemical-related effects on mortality, clinical chemistry observations, urinalysis,gross/microscopic (including ophthalmologic) observations.

 

Rats:

At≥382 ppm: increased liver weight.

At 782 ppm: haematology changes (small increases in leukocyte counts (males/females); increases in platelet counts (males)).

 

Mice:

At≥382 ppm:increased absolute and relative liver weights (females);

At 782 ppm: increased relative liver-to-brain weight ratios (males).

Rabbits:

At 1610 ppm: reduced body weight (females)

 

 

Applicant's summary and conclusion

Conclusions:
The LOEL of 382 ppm was reported in rats and mice and LOEL of 1610 ppm was reported in rabbits.