Butanone

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP, not according to current standart test protocols

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Inhalation risk test; equivalent to H.F. Smyth et al; 1962; Am. Ind. Hyg. Ass. J. 23, 95-107

GLP compliance:

no

Test type:

other: Inhalation risk test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butanone

EC Number:

201-159-0

EC Name:

Butanone

Cas Number:

78-93-3

Molecular formula:

C4H8O

IUPAC Name:

butan-2-one

Details on test material:

- Name of test material (as cited in study report): Methylethylketone

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation:
10 minutes exposure group: 228g (males); 199g (females)
30 minutes exposure group: 218g (males); 189g (females)
1 hour exposure group: 238g (males); 174g (females)
1.2 hour exposure group: 233g (males); 179g (females)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- A saturated atmosphere was generated by bubbling 200l/h air at 20 ° C through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 10 min, 30 min, 1h or 1.2h.

TEST ATMOSPHERE
- No analytical determination of the atmosphere concentration was performed. The nominal concentration was calculated as the quotient of the amount of test substance weight loss during the exposure and the amount of air used during the exposure.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

<= 1.2 h

Remarks on duration:

10 min, 30 min, 1h, 1.2h tested

Concentrations:

10 minutes exposure group: appr. 330 mg/l
30 minutes exposure group: appr. 230 mg/l
1 hour exposure group: appr. 250 mg/l
1.2 hour exposure group: appr. 220 mg/l

No. of animals per sex per dose:

10 minutes exposure group: 6
30 minutes exposure group: 9
1 hour exposure group: appr. 3
1.2 hour exposure group: appr. 3

Control animals:

other: not applicable

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Mortality:

10 minutes exposure group: 0/12
30 minutes exposure group: 2/18
1 hour exposure group: 1/6
1.2 hour exposure group: 3/6

Clinical signs:

other: Attempts to escape; accelerated to intermittent respiration; eyelid closure, salivation, eye secretion with slight chromodacryorrhea; loss of pain reflex; lateral position; narcotic state, slow gait, ataxia, apathy, ruffled fur.

Body weight:

No data

Gross pathology:

Found dead:
Heart (acute dilatation, acute congestion hyperemia);
Lung (partial slight to medium acute exhalation; partly single cranial parts were carnified);
Liver (single bright parts, clay coloured discoloration)
sacrificed scheduled:
organs without findings