Registration Dossier
Registration Dossier
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EC number: 201-159-0 | CAS number: 78-93-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest
- Author:
- Bringmann G. & Kuehn R.
- Year:
- 1 977
- Bibliographic source:
- Z Wasser Abwasser Forsch 10(3/4): 87-98
- Reference Type:
- publication
- Title:
- Grenzwerte der Schadwirkung wassergefaehrdender Stoffe gegen Blaualgen (Microcystis aeruginosa) und Gruenalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest.
- Author:
- Bringmann G. & Kuehn R.
- Year:
- 1 978
- Bibliographic source:
- Vom Wasser 50: 45-60
- Reference Type:
- publication
- Title:
- Testing of substances for their toxicity threshold: Model organisms Microcystis (Diplocystis) aeruginosa and Scenedesmus quadricauda.
- Author:
- Bringmann G. & Kuehn R.
- Year:
- 1 978
- Bibliographic source:
- Mitt Internat Verin Limnol 21: 275-284.
- Reference Type:
- publication
- Title:
- Comparison of the Toxicity Thresholds of Water Pollutants to Bacteria, Algae and Protozoa in Cell Multiplication Inhibition Test
- Author:
- Bringmann G. & Kuehn R.
- Year:
- 1 980
- Bibliographic source:
- Water Research 14(3): 231-241
Materials and methods
- Principles of method if other than guideline:
- cell growth inhibition test
- GLP compliance:
- no
Test material
- Reference substance name:
- Butanone
- EC Number:
- 201-159-0
- EC Name:
- Butanone
- Cas Number:
- 78-93-3
- Molecular formula:
- C4H8O
- IUPAC Name:
- butan-2-one
- Details on test material:
- - Name of test material (as cited in study report): Äthylmethylketon
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Scenedesmus quadricauda
- Details on test organisms:
- BREEDING OF TEST ORGANISMS
- 100 ml Erlenmeyer flask with 20 ml nutrient solution
- relative humidity 50 %
- renewal of preparatory culture every 10 days
- temperature 27 °C
- illuminated permanent and protected against daylight
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 d
Test conditions
- Test temperature:
- 27°C
- pH:
- adjusted to pH 7
- Details on test conditions:
- TEST PROCEDURE
- test vessels: Kabsenberg test tubes
- test volume: 10 ml
- daily shaken
- cell culture: 5 ml algae suspension per test flask (turbidity 20 formazine nephelometric units)
- number of replicates: 3
- measurement of extinction: Hg 578 nm
- test criterion: cell multiplication inhibition (calculated by measuring the extinction)
Results and discussion
Effect concentrations
- Duration:
- 8 d
- Dose descriptor:
- other: TGK
- Effect conc.:
- 4 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: turbidity (cell multiplication)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
