Hydrocarbons, C18-C22, n-alkanes, n-alkenes

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none
- Analysis of the freshly prepared test media at 0 and 48 hours showed measured test concentrations of 1.79 and 1.86 mg/L respectively.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50/LC50: 24h: 1.3 mg/L, 48h: 1.1 mg/L (95% confidence limits: 1.0 - 1.8 mg/L)
- Other: exposure conditions were similar to those in the definitive test

Result:

Cumulative Immobilization Data in the Definitive Test
Nominal Concentration (mg/L)	Cumulative Immobilized Daphnia (Initial Population: 5 Per Replicate)
	24 Hours						48 Hours
	R1	R2	R3	R4	Total	%	R1	R2	R3	R4	Total	%
Control	0	1*	0	0	1	5	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0	0	0
R1– R4= Replicates 1 to 4 * Single immobilized daphnid considered to be due to natural causes as less than 10% immobilization occurred

Validity criteria fulfilled:

yes

Conclusions:

In a reliable 48 -h acute toxicity immobilization study Daphnia were exposed to Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes at measured WAF of 100 mg/L under static conditions following OECD 202 guidelines.  The 48 - h EL50was > 100 mg/L, based on mobility.

Executive summary:

In a reliable 48 -h acute toxicity immobilization study Daphnia were exposed to Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes at measured WAF of 100 mg/L under static conditions following OECD 202 guidelines.  The 48 - h EL₅₀was > 100 mg/L, based on mobility.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In a reliable 48 hour acute toxicity immobilization study Daphnia were exposed to Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes at measured WAF of 100 mg/L under static conditions following OECD 202 guidelines.  The 48 hour EL₅₀was > 100 mg/L, based on mobility. No toxicity was observed at the level of water solubility.