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EC number: 603-290-2
CAS number: 128625-52-5
of cy steine and lysine % depletion
Test item Art. 851009
Peak areareferencecontrol C
Replicate 1Replicate 2Replicate 3
SD % depletion Cysteine < 14.9 met:SD % depletion Lysine < 11.6 met:
Meandepletion(Cysteine + Lysine)8.85
ReactivityClass(Cysteine + Lysine)LOW
CV = coefficient of variation
of cysteine and lysine % depletion (continued)
Positive control: Cinnamic aldehyde, vehicle: acetonitrile
60.8 < Mean % depletion Cysteine < 100
40.2< Mean % depletion Lysine < 69.0
SD % depletion Cysteine < 14.9
SD % depletion
Lysine < 11.6
study was conducted according to OECD TG 442C in order to evaluate the
reactivity of the test item towards cysteine (Cys-) and lysine (Lys-)
containing peptides. The
DPRA is an in chemico method which quantifies the remaining
concentration of cysteine- or lysine-containing peptide following 24 ± 2
hours incubation with the test item at 25 ± 2.5°C. The test item was
dissolved at a concentration of 100 mM in acetonitrile. Relative peptide
concentration is measured by high-performance liquid chromatography
(HPLC) with gradient elution and UV detection at 220 nm.
aldehyde was used as positive control at a concentration of 100 mM in
acetonitrile. Treatment with the positive control item revealed a
cysteine and lysine peptide depletion of 69.85% for cysteine and 56.79%
for lysine peptide. These values are within the required range of 60.8%
and 100% for the cysteine peptide and between 40.2% and 69.0% for the
item-treated samples revealed a cysteine peptide depletion of 6.55% and
a lysine peptide depletion of 11.14% (mean peptide depletion of 8.85%)
and, hence, were below 22.62% and above 6.38%. The test item is
considered positive and predicted to be a sensitiser (low reactivity) in
the Direct Peptide Reactivity Assay (DPRA).
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