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EC number: 949-790-7
CAS number: -
TABLE 1 - Number of introduced Daphnids
and Incidence of immobility
TABLE 2 - POSITIVE CONTROL
Acceptability of the Test:
1. In the control, no daphnids
became immobilised or showed other signs of disease or stress such as
discoloration or unusual behaviour such as trapping at the surface of
2. The dissolved oxygen
concentration at the end of the test was ≥3 mg/L in control and test
All results presented in the tables of the
report are calculated using values as per the raw data rounding
procedure and may not be exactly reproduced from the individual data
The average exposure concentrations were
calculated as , being the geometric means of the concentrations of
Esacure 3644 measured in the samples taken at the start (Ct=0) and the
end of the test (Ct=48).
No EC50 could be calculated because the
test item proved to be non-toxic (EC50 > maximum soluble concentration
No EL50 could be calculated because the
test item proved to be non-toxic (EL50 > maximum loading rate tested).
Critical computerized systems used in the
study are listed below or presented in the appropriate Phase Report.
All computerized systems used in the conduct of this study have been
validated; when a particular system has not satisfied all requirements,
appropriate administrative and procedural controls were implemented to
assure the quality and integrity of data.
The objective of the study was to evaluate
Esacure 3644 for its ability to generate acute toxic effects on the
mobility of Daphnia magna during an exposure period of 48 hours and, if
possible, to determine the EC50 at 24 and 48 hours of exposure.
The study procedures described in this
report were based on the OECD guideline No. 202, 2004. In addition,
procedures were based on the test methods described in the OECD series
on testing and assessment number 23, 2019.
The batch of Esacure 3644 tested was a
light yellow solid UVCB and not completely soluble in test medium at the
loading rates initially prepared.
Water Soluble Fractions (WSFs) were
individually prepared at loading rates ranging between 1.0 and 100 mg/L
and used as test concentrations. Preparation of test solutions was
performed under dimmed light conditions and glassware used was wrapped
in aluminium foil to minimize exposure to light.
A combined limit/range-finding test was
performed. Twenty daphnids per group (5 per replicate, quadruplicate)
were exposed to an untreated control and to a WSF prepared at a loading
rate of 100 mg/L, in a limit test. In addition ten daphnids per group (5
per replicate, duplicate) were exposed to WSFs individually prepared at
loading rates of 1.0 and 10 mg/L in the combined range-finding test. The
total exposure period was 48 hours and samples for analytical
confirmation of exposure concentrations were taken at the start and at
the end of the test. Since the test item was expected to be sensitive to
light, the exposure was performed in the dark.
No immobility was observed in the control
and at any of the test concentrations throughout the exposure period.
In the limit concentration, 6.3 and 4.3
µg/L were measured at the start and end of the test, respectively.
Accordingly, the average exposure concentration was calculated to be 5.2
The study met the acceptability criteria
prescribed by the study plan and was considered valid.
In conclusion, the 48h-EC50 for Daphnia
magna exposed to Esacure 3644 exceeded the maximum solubility of the
test item in test medium at a loading rate of 100 mg/L, i.e. exceeded an
average exposure concentration of 5.2 µg/L. The 48h-EL50 for Daphnia
magna exposed to Esacure 3644 was beyond the range tested, i.e. exceeded
a loading rate of 100 mg/L.
Study performed to recognised OECD
guidlines with GLP certification
The 48h-EC50 for Daphnia magna exposed to
Esacure 3644 exceeded the maximum solubility of the test item in test
medium at a loading rate of 100 mg/L, i.e. exceeded an average exposure
concentration of 5.2 µg/L
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