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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
December 1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
- Principle of test: 1-hr inhalation exposure
- Short description of test conditions: rats are exposed for 1 hour to 2 mg/L air and then observed during 14 days
- Parameters analysed / observed: clinical signs, mortality, body weight, autopsie
GLP compliance:
no
Test type:
fixed concentration procedure

Test material

Constituent 1
Chemical structure
Reference substance name:
2-thienylacetyl chloride
EC Number:
254-290-0
EC Name:
2-thienylacetyl chloride
Cas Number:
39098-97-0
Molecular formula:
C6H5ClOS
IUPAC Name:
2-thienylacetyl chloride
Test material form:
liquid
Details on test material:
- Density: 1.29

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data
- Females (if applicable) nulliparous and non-pregnant: no data
- Age at study initiation: no data
- Weight at study initiation: 202.8g for males and 190.2g for females on D0 (mean value)
- Fasting period before study: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes: around 10/hours
- Photoperiod (hrs dark / hrs light): no data

IN-LIFE DATES: no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
A parallelepipedic glass vessel, 100L in volume, is continuously crossed by the atmosphere overloaded with the product. These vapors are obtained by creating a vaporization of the product inside a glass column: this column is fed, on the one hand, with compressed air at a known and adjustable flow rate and, on the other hand, with the liquid substance also arriving at a determined rate by means of a syringe infuser (Perfusor Braun).
This 100L vessel is crossed by the overloaded atmosphere with a flow rate of approximately 1 m3 per hour. A ventilator located inside the vessel homogenises continuously the atmosphere.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
1 h
Concentrations:
2.05 mg/L
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing D0, 2, 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 2.05 mg/L air
Based on:
test mat.
Exp. duration:
1 h
Sex:
male/female
Dose descriptor:
LC0
Effect level:
>= 2.05 mg/L air
Based on:
test mat.
Exp. duration:
1 h
Mortality:
no mortality
Clinical signs:
other: during the exposure: attempted escape, hyperactivity, closing of the palpebral fissure, sneezing, trembling, loose breathing movements. after the exposure: tachycardia (D0-1), hyperactivity (D0-4), then normal behavior.
Body weight:
no effect
Gross pathology:
whitish mottling and numerous red spots in the lungs in several animals.
gastric mucous irritation in one female.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Conclusions:
In this study in rats the 1-hr LC0 is 2.05 mg/L (no mortality is observed).
Executive summary:

In an acute inhalation toxicity study, groups of Sprague-Dawley rats (5/sex) were exposed by inhalation route to 2-thienylacetyl chloride for 1 hour (whole body) at concentration of 2.05 mg/L.  Animals then were observed for 14 days.

LC0 Combined = 2.05 mg/L (no mortality occurred)