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EC number: 828-365-9
CAS number: 113573-69-6
The pile ignited briefly with an orange flame which self-extinguished
without propagating combustion.
Since the test item did not propagate combustion along 200 mm of the
powder train within the four minutes test period, the test item is not
considered to be highly flammable and no further testing was required.
Cellulase, Batch PPC31776 (freeze-dried aliquot) has been determined to
be not highly flammable, using a procedure designed to be compatible
with Method A.10 Flammability (Solids) of Commission Regulation (EC) No
440/2008 of 30 May 2008.
Cellulase was freeze-dried and tested for flammability. The test showed
that cellulase is not flammable.
Most of the enzymes are produced by fermentation, typically in solution.
After the fermentation, enzymes are recovered as enzyme concentrate in a
liquid form and further formulated as liquid products or granules.
The substance in contact with water is not considered to be highly
flammable according to Regulation (EC) No 440/2008.
Based on this data, other enzymes are also expected to be Non-flammable
Enzymes are globular proteins produced by fermentation i.e. typically in
solution. After the fermentation, enzymes are recovered as enzyme
concentrate in a liquid form and further formulated as liquid products
or granules. The production process is described in Section 1.2 of
IUCLID in more details. Decades of experience in production, handling
and use of enzymes show that the substance does not react with water
(e.g. the substance is manufactured with water or/and washed with
water). The substance is soluble (see Section 4.8 of IUCLID) and stable
in water. Therefore, the substance in contact with water is not
considered to be highly flammable according to Regulation (EC) No
440/2008. Under REACH, enzymes are defined as enzyme concentrate, dry
matter (1). As dry matter, proteins in general are not considered to be
highly flammable according to Regulation (EC) No 440/2008. This is
supported by the chemical structure of the proteins. Proteins contain
reactive groups such as hydroxyl, carboxylic acid, amines, thiols groups
etc. During combustion, carboxylic acid groups may go through
decarboxylation and hydroxyl groups may be released as water vapor (3).
The carboxylic acid may also promote char formation (2, 3). Proteins
also contain nitrogen and sulfur which form a disulfide bond helping in
contributing to its non-inherent flammability (2, 3). These properties
have in fact led to the use of proteins as flame retardants (2, 3).
Additionally, several proteins have already been tested using recognized
method under REACH and CLP (e.g. UN Test N.1) and found not to be highly
flammable according to Regulation (EC) No 440/2008, e.g. Protein
hydrolyzates, rice bran (EC number: 305-224-5), Protein hydrolyzates,
animal (EC number: 309-203-1), Insulin DesB30 (EC number: 944-550-8).
References: 1. Guidance for identification and naming of substances
under REACH and CLP, Version 2.1.
3. https://patents.google.com/patent/WO2000029662A1/en Based on above
weight of evidence, a study for flammability is deemed unnecessary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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