2-hydroxy-3-methylcyclopent-2-enone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-3-methylcyclopent-2-enone

EC Number:

201-303-2

EC Name:

2-hydroxy-3-methylcyclopent-2-enone

Cas Number:

80-71-7

Molecular formula:

C6H8O2

IUPAC Name:

2-hydroxy-3-methylcyclopent-2-en-1-one

Specific details on test material used for the study:

Test Item
Designation in Test Facility: Methyl Cyclopentenolone
Date of Receipt: 14. Jul. 2017
Condition at Receipt: Room temperature, in proper conditions
Specification
The following information concerning identity and composition of the test item was pro-vided by the sponsor.
Name Methyl Cyclopentenolone
Batch no. D270001706002
Appearance white, crystal powder
Composition Methyl Cyclopentenolone
CAS No. 80-71-7
EINECS-No. 201-303-2
Molecular formula C6H8O2
Molecular weight 112.13 g/mol
Purity >99.0%
Homogeneity homogeneous
Vapour pressure unknown
Stability H2O: unknown; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Solubility H2O: < 0.1 g/L; EtOH: >1 g/L; acetone: >1 g/L;
CH3CN: >1 g/L; DMSO: >1 g/L
Production date 08. Jun. 2017
Expiry date 07. Jun. 2018
Storage Room Temperature (20 ± 5°C)
Storage in Test Facility
The test item was stored in the test facility in a closed vessel at room temperature (20 ± 5°C) protected from light and humidity.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge was used as inoculum.
Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 25. Oct. 2017, batch no: 20171025
The sludge was filtrated, washed with medium 2x and re-suspended in test medium. It was then aerated.
The dry matter was determined as 4560 mg suspended solids/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

74

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The degree of biodegradation reached 74% after 28 days
The degradation phase was from day 8 – day 17 (67%).
The 10-day-window began on day 8 (this is stated as lag phase), at its end (day 18), 69 % were reached, surpassing the pass level of 60 % given in the OECD guideline.
Therefore, when applying the 10-day-window, the test item Methyl Cyclopentenolone is readily biodegradable following OECD 301F/EU C.4-D.