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EC number: 202-795-1
CAS number: 99-86-5
Table 1. Migration of nuclear DNA from organs of mice treated with
Table 2. Migration of nuclear DNA from organs of mice treated with
In an in vivo comet assay, 4 male ddY mice were administered a single
oral dose of d-limonene in olive oil by gavage at dose levels of 2000
mg/kg bw. Animals were then observed for pharmacotoxic signs and were
macroscopically necropsied 3, 8 and 24 hours after treatment. Stomach,
colon, liver, kidney, urinary bladder, lung, brain and bone marrow were
isolated and the prepared slides were scanned to determine the length of
the whole comet, diameter of the head and mean migration of 50 nuclei
per organ per animal. A preliminary range-finding test was also
conducted using 4-5 male mice/dose to determine the LD50 value. No
death, morbidity or distinctive clinical and microscopic signs were
observed. D-limonene did not induced DNA damage in the studied organs.
Under the test conditions, d-limonene is not considered as mutagenic in
the comet assay and does not need to be classified according to the
criteria of the CLP Regulation (EC) N° (1272-2008).
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