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EC number: 221-309-9
CAS number: 3063-94-3
2,2,2-trifluoro-1-(trifluoromethyl)ethyl methacrylate was tested
Table1 summarizes the opacity, permeability andin vitroirritancy
scores of the test item and the controls. The opacity, permeability and
in vitro scores of the individual corneas are shown in Table 2- 5.
The individual in vitro irritancy scores for the negative controls
ranged from 0.3 to 2.3. The individual positive control in vitro
irritancy scores ranged from 37 to 53. The corneas treated with the
positive control item were turbid after the 10 minutes of treatment.
The corneas treated with the test item showed opacity values
ranging from -0.8 to -0.4 and permeability values ranging from 0.001 to
0.003. The corneas were clear after the 10 minutes of treatment with the
test item. No pH effect of the test item was observed on the rinsing
medium. Hence, the in vitro irritancy scores ranged from -0.8 to -0.3
after 10 minutes of treatment with
Table 1: Summary of Opacity, Permeability and In Vitro Scores
using the negative control mean opacity and mean permeability values for
the positive control and test item.
vitro irritancy score (IVIS) = mean opacity value + (15 x mean OD490
Table 2: Opacity Scores
Opacity = Opacity after treatment – Opacity before treatment.
2 Negative control
corrected Final Opacity = Final opacity – Mean final opacity negative
3 Calculations are
made without rounding off.
Table 3: Permeability Score individual Values (Uncorrected)
Table 4: Permeability Score Individual Values (corrected)
1 OD490 values
corrected for the mean final negative control permeability (-0.004).
2 Calculations are
made without rounding off.
Table 5: In Vitro Irritancy Score
1 In vitro irritancy score (IVIS) =
opacity value + (15 x OD490 value).
2 Positive control and test item are
corrected for the negative control.
The objective of this study was to evaluate the eye hazard
potential of 2,2,2-trifluoro-1-(trifluoromethyl)ethyl methacrylate as
measured by its ability to induce opacity and increase permeability in
an isolated bovine cornea using the Bovine Corneal Opacity and
Permeability test (BCOP test).
This report describes the potency of chemicals to induce serious
eye damage using isolated bovine corneas. The eye damage of the test
item was tested through topical application for 10 minutes.
The study procedures described in this report were based on the
most recent OECD guideline.
Batch 449935-248 of the test item was a clear colourless
liquid. The test item was applied as it is (750 µl) directly on top of
The negative control responses for opacity and permeability were
less than the upper limits of the laboratory historical rangeindicating
that the negative control did not induce irritancy on the corneas. The
mean in vitro irritancy score of the positive control (Ethanol) was 48
and was within two standard deviations of the current historical
positive control mean. It was therefore concluded that the test
conditions were adequate and that the test system functioned properly.
The test item did not induce ocular irritation through both
endpoints, resulting in a mean in vitro irritancy score of -0.5 after
10 minutes of treatment.
conclusion, since 2,2,2-trifluoro-1-(trifluoromethyl)ethyl methacrylate
induced an IVIS ≤
3, no classification is required for eye irritation or serious eye
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