Anisoyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviwed journal

Data source

Reference

Reference Type:

review article or handbook

Title:

AMES test for p-Anisaldehyde

Author:

National Institute of Technology and Evaluation.

Year:

2017

Bibliographic source:

Japan chemicals collaborative knowledge database (J-check), 2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

To evaluate the mutagenic potential of p-Anisaldehyde in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia
coli WP2 uvrA.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Anisaldehyde

EC Number:

204-602-6

EC Name:

Anisaldehyde

Cas Number:

123-11-5

Molecular formula:

C8H8O2

IUPAC Name:

Anisaldehyde

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Anisic aldehyde/ p-Anisaldehyde
- Molecular formula: C8H8O2
- Molecular weight: 136.1492 g/mol
- Substance type: organic
- Physical state: liquid
-Smiles: COc1ccc(C=O)cc1
-InChI: 1S/C8H8O2/c1-10-8-4-2-7(6-9)3-5-8/h2-6H,1H3

Specific details on test material used for the study:

Details on test material
- Name of test material (as cited in study report): 4-Methoxybenzaldehyde
- Molecular formula: C8H8O2
- Molecular weight: 136.1492g/mol
- Substance type: organic

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

bacteria, other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.

Details on mammalian cell type (if applicable):

Not applicable.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9from Rat liver, induced with phenobarbital and 5,6-benzoflavone

Test concentrations with justification for top dose:

-S9 mix; 39.1, 78.1, 156, 313, 625, 1250 µg/plate(TA100, TA1535,
TA1537)
-S9 mix; 78.1, 156, 313, 625, 1250, 2500, 5000 µg/plate(WP2 uvrA)
-S9 mix; 156, 313, 625, 1250, 2500, 5000 g/µplate(TA98)
+S9 mix; 156, 313, 625, 1250, 2500, 5000 µg/plate(TA100, TA1535,
TA98, TA1537)
+S9 mix; 39.1, 78.1, 156, 313, 625, 1250, 2500, 5000 µg/plate(WP2
uvrA)

Vehicle / solvent:

Vehicle
- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: -S9 mix; 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, TA98 and WP2 uvrA), Sodium azide (TA1535) and 9-Aminoacridine hydrochloride (TA1537) +S9 mix; 2-Aminoanthracene (all strains)

Details on test system and experimental conditions:

Details on test system and conditions
METHOD OF APPLICATION: Pre-incubation method
NUMBER OF REPLICATIONS: 2
- Other: 3 plates per test were observed.

Rationale for test conditions:

Not specified.

Evaluation criteria:

The test was considered to be positive, if there is increase in revertant colonies.

Statistics:

Not specified

Results and discussion

Test results

Species / strain:

bacteria, other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Remarks on result:

other: No mutagenic effect were observed.

Applicant's summary and conclusion

Conclusions:

4-Methoxybenzaldehyde(123-11-5) was evaluated for its mutagenic potential in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA. The test result was considered to be negative in the presence and absence of metabolic activation.

Executive summary:

Genetic toxicity in vitro study for p-Anisaldehyde was assessed for its possible mutagenic potential. For this purpose AMES test was performed according to Guidelines for Screening Mutagenicity Testing of Chemicals(Chemical Substances Control Law of Japan) and OECD Test Guideline 471 .The test material was exposed to Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvr A at different concentration in the presence and absence of metabolic activation. No mutagenic effects were observed. Hence p-Anisaldehyde was considered to be non mutagenic in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA by AMES test .Hencethe test substance cannot be classified as gene mutant in vitro.