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EC number: 219-948-3
CAS number: 2580-77-0
Inhibition of Growth Rate and Yield in the Definitive Test:
Growth Rate (cells/mL/hour)
0 – 72 h
* In accordance with the OECD test guideline only the
mean value for yield for each test concentration is calculated
R1-R6= Replicates 1 to 6
SD= Standard Deviation
in growth as compared to controls]
A study was performed to assess the effect of the test item on the
growth of the green alga Pseudokirchneriella subcapitata. The
method followed was designed to be compatible with the OECD Guidelines
for Testing of Chemicals (2006) No 201, "Freshwater Alga and
Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of
Commission Regulation (EC) No 761/2009.
Following a preliminary range-finding test, Pseudokirchneriella
subcapitata was exposed to an aqueous solution of the test item at a
concentration of 100 mg/L (six replicate flasks) for 72 hours, under
constant illumination and shaking at a temperature of 24 ± 1 °C.
Samples of the algal populations were removed daily and cell
concentrations determined for each control and treatment group, using a
Coulter®Multisizer Particle Counter.
Analysis of the test preparations at 0 and 72 hours showed measured test
concentrations to be near nominal and so the results are based on
nominal test concentrations only.
Exposure of Pseudokirchneriella subcapitata to the test item gave
EC50values of greater than 100 mg/L and correspondingly the
No Observed Effect Concentration was 100 mg/L.
It was considered unnecessary and unrealistic to test at concentrations
in excess of 100 mg/L.
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