2,4-xylidine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981-08-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Deviations to current guideline: Shortened test duration (48 instead of 96h),. The test was conducted following an early draft of DIN 38 412 Part 15.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Shortened test duration (48 instead of 96h),. The test was conducted following an early draft of DIN 38 412 Part 15.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,4-xylidine

EC Number:

202-440-0

EC Name:

2,4-xylidine

Cas Number:

95-68-1

Molecular formula:

C8H11N

IUPAC Name:

2,4-dimethylaniline

Test solutions

Vehicle:

no

Details on test solutions:

- Stock solution: 10 g/L in de-ionized water, stirred over a period of 24 hours at room-temperature (approx. 20 - 22 °C) and a pH of 2.3.

26200 mg chemical oxygen demand / L (COD)
- Solubility in water: Not mentioned in the laboratory manuscript.
- Homogeneity and stability in aqueous solution: A precipitation of a sticky solid substance occurs during storage of the stock solution.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Origin and breeding: not mentioned in the laboratory manuscript.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no data

Test conditions

Test temperature:

20-22°C

Salinity:

fresh water

Nominal and measured concentrations:

Analyses of the stock solution: 7568 mg dissolved organic carbon / L (DOC)
(calculated value: 7929 mg C / g)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 50 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The LC100 was determined as the lowest tested concentration with a letal effect of 100 %.
The LC50 was determined to be beteween 50 and 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

NOEC (48 hours) is 50 mg/L. LC100 (48hours) is 100 mg/L. was reduced due to 100 % lethal effect after 48 h.