1-hydroxybenzotriazole

Endpoint summary

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with 24 hrs continuous stirring. After the completion of 24 hrs, stock solution was analytically detected and from this stock solution required test solution was prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 100 mg/l was prepared by dissolving white powder  in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.3.0 ,  6.0 , 12.0, 25.0, 50.0, 100.0 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

  The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 75.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving white powder in OECD growth medium . Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration used were 4.1 , 9.0 , 20.0 , 45.0 , 100 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance , in algae was determined to be 87.3 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganism:

Data available for the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical.The studies are as mentioned below:

In first study ,inhibitory effects of test chemical on micro organism species Bacillus spp. was observed using different soil and water samples as inoculum. Test chemical analytically monitorized by thin-layer chromatography. A stock solution (0.276 mg/ml) was prepared in methanol,with an activity of 36 512 dpm/ul). Methanol were used as a vehicle in which test chemical dissolved. Test conducted under the static system for 192 hrs. During experiment the Effect of benzimidazole derivatives on maximum growth of Bacillus strains was observed to be at concentrations 500-1000 mg/l (reported as ug/ml) using tubes shaken in water bath at 30C; Klett-Summerson readings taken at intervals up to 192 h after inoculation. Based on the EC50 value, chemical consider to be nontoxic.

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with 24 hrs continuous stirring. After the completion of 24 hrs, stock solution was analytically detected and from this stock solution required test solution was prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

Data two experimental reports were used to determine Short term toxicity to aquatic invertebrate.

In the first experimental reportaim of the study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 100 mg/l was prepared by dissolving white powder  in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.3.0 ,  6.0 , 12.0, 25.0, 50.0, 100.0 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

  The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 75.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

In secondexperimental report , daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material.

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 10 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.

The nominal concentration selected for the experiment was 100 mg/l and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be 100 mg/l. immobility of daphnia was observed .Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibits short term toxicity to Daphnia.

After 48 hours of exposure to test item to nominal test concentrations, EC50 was determine to be 100 mg/l . Based on the EC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving white powder in OECD growth medium . Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration used were 4.1 , 9.0 , 20.0 , 45.0 , 100 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance , in algae was determined to be 87.3 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganism:

Data available for the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical.The studies are as mentioned below:

In first study ,inhibitory effects of test chemical on micro organism species Bacillus spp. was observed using different soil and water samples as inoculum. Test chemical analytically monitorized by thin-layer chromatography. A stock solution (0.276 mg/ml) was prepared in methanol,with an activity of 36 512 dpm/ul). Methanol were used as a vehicle in which test chemical dissolved. Test conducted under the static system for 192 hrs. During experiment the Effect of benzimidazole derivatives on maximum growth of Bacillus strains was observed to be at concentrations 500-1000 mg/l (reported as ug/ml) using tubes shaken in water bath at 30C; Klett-Summerson readings taken at intervals up to 192 h after inoculation. Based on the EC50 value, chemical consider to be nontoxic.

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and