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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Title:
No information
Author:
Mortelmans K et al
Year:
1986
Bibliographic source:
Environ Mutagen 8:1-119 (1986)

Materials and methods

Test guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzoin
EC Number:
204-331-3
EC Name:
Benzoin
Cas Number:
119-53-9
Molecular formula:
C14H12O2
IUPAC Name:
2-hydroxy-1,2-diphenylethanone

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
rat and hamster S9 fraction
Test concentrations with justification for top dose:
33 to 3333 microgram/plate

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Additional information on results:
Benzoin was found to be negative when tested for mutagenicity using the
Salmonella/microsome preincubation assay, using the standard protocol
approved by the National Toxicology Program (NTP). Benzoin was tested in
as many as 6 Salmonella typhimurium strains (TA1535, TA1537, TA 1538, TA97, TA98,
and TA100) in the presence and absence of rat and hamster liver S-9, at
doses of 0.033, 0.0333, 0.100, 0.333, 0.3333, 0.667, 1.000, and 3.3333
mg/plate. The highest ineffective dose tested in any S. typhimurium strain
was 3.3333 mg/plate. Precipitate was observed in most cultures at the high
dose and also some clearing of the background bacterial lawn was also
seen. [Mortelmans K et al; Environ Mutagen 8:1-119 (1986)]
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion