Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: -
CAS number: -
Results after 240 Minutes Incubation Time
Opacity value = Difference (t240-t0) of Opacity
Permeability at 490 nm (OD490)
Proposedin vitroIrritation Scale
FAT 40842/A TE
10% (w/v) Benzalconium chloride in 0.9% (w/v) NaCl (physiological salt
solution, produced in-house) served as positive control.
0.9% (w/v) NaCl (physiological salt solution, produced in-house) served
as negative control.
Proposedin vitroirritation Scale
0 - 3
Non eye irritant
3.1 – 25
Mild eye irritant
25.1 – 55
Moderate eye irritant
55.1 – 80
Severe eye irritant
Very severe eye irritant
This in vitro study was performed to assess the corneal irritation and
damage potential of FAT 40849/A TE by means of the BCOP assay using
fresh bovine corneae.
After a first opacity measurement of the fresh bovine corneae (t0), each
about 100 mg of the test item FAT 40849/A TE, 1 mL of the positive
control, and also 1 mL of the negative control were applied to the
corneae and incubated for 240 minutes at 32 ± 2 °C in complete medium
(cMEM medium supplemented with sodium bicarbonate and Lglutamine and 1%
fetal calf serum (FCS)). After the incubation phase the test item, the
positive, and the negative controls were each rinsed from the corneae
and opacity was measured again (t240).
After the opacity measurements permeability of the corneae was
determined while application of 1 mL of a fluorescein solution for about
90 minutes at 32 ± 2 °C in a horizontal position. The coming out liquid
was measured spectrophotometrically.
With the negative control (0.9% NaCl solution) neither an increase of
opacity nor permeability of the corneae could be observed. The mean in
vitro score was calculated as 2.59.
The positive control (10% (w/v) Benzalconium chloride) showed clear
opacity and distinctive permeability of the corneae and therefore, is
classified as very severe eye irritant. The mean in vitro score was
calculated as 221.84.
The test item FAT 40849/A TE caused clear opacity and permeability of
the corneae compared with the results of the negative control. The
calculated mean in vitro score was 457.95 and therefore, the test item
was classified as very severe eye irritant.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Tato webová stránka používá cookies, aby se vám naše stránky používaly co nejlépe.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again